caldicellulosiruptor_medium_with_2_5_nacl

CultureMech:010422 bacterial COMPLEX LIQUID
Original name: Caldicellulosiruptor Medium With 2.5% NaCl

Overview

External ontology
TOGO:M994 — Caldicellulosiruptor Medium With 2.5% NaCl
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M994 Original source: JCM - JCM_M947 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=947

Composition

flowchart LR
    CultureMech_010422["caldicellulosiruptor_medium_with_2_5_nacl"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010422 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010422 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_010422 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_010422 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010422 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_010422 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_010422 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010422 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_FeCl3_6H2O["FeCl3・6H2O"]
    CultureMech_010422 --> ing8_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing8_FeCl3_6H2O -.-> CHEBI_86254
    ing9_Cellobiose["Cellobiose"]
    CultureMech_010422 --> ing9_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing9_Cellobiose -.-> CHEBI_17057
    ing10_Glucose["Glucose"]
    CultureMech_010422 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_010422 --> ing11_Trypticase_peptone__BD_BBL_
    ing12_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010422 --> ing12_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing13_N2["N2"]
    CultureMech_010422 --> ing13_N2
    CHEBI_17997["CHEBI:17997"]
    ing13_N2 -.-> CHEBI_17997

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.75 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.9 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6 K2HPO4
HO4P.2K 174.174 g/mol
1.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
8 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
9 FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
2.5 G_PER_L CHEBI:86254
iron trichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 Cellobiose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17057
cellobiose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
11 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
12 Trypticase peptone (BD-BBL) 2 G_PER_L Properties: Undefined component
13 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.75 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
14 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.107587Z togo-import Imported from TOGO Medium Source: JCM, ID: M994
2026-01-28T19:48:35.705228Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:59.434203+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:13.759770Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010422
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.735993+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Caldicellulosiruptor Medium With 2.5% NaCl" to "caldicellulosiruptor_medium_with_2_5_nacl" for consistent matching
2026-05-16T09:03:22.643941+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:46.385199+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.272389+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.061683+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 18cd27846f9267249dd5b9b7a2f6ba4d57876d7481947e78eea766798b1b9927