Overview
- External ontology
- TOGO:M997 — NH-JN4 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M997 Original source: JCM - JCM_M950 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=950
Composition
flowchart LR
CultureMech_010425["nh_jn4_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010425 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010425 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010425 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_010425 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_010425 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_010425 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_010425 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010425 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_010425 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_010425 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Na2SO4["Na2SO4"]
CultureMech_010425 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11__NH4_2Fe_SO4_2_6H2O["(NH4)2Fe(SO4)2・6H2O"]
CultureMech_010425 --> ing11__NH4_2Fe_SO4_2_6H2O
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010425 --> ing12_Yeast_extract__BD_Difco_
ing13_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010425 --> ing13_Tryptone__BD_Difco_
ing14_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_010425 --> ing14_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010425 --> ing15_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing15_Carbon_dioxide_gas -.-> CHEBI_16526
ing16_Nitrogen_gas["Nitrogen gas"]
CultureMech_010425 --> ing16_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing16_Nitrogen_gas -.-> CHEBI_17997
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
960 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
6.8 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Simple component, Defined component, Organic compound |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 | (NH4)2Fe(SO4)2・6H2O | 2 G_PER_L | Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound | |
| 13 | Yeast extract (BD-Difco) | 3 G_PER_L | Properties: Undefined component | |
| 14 | Tryptone (BD-Difco) | 4 G_PER_L | Properties: Undefined component | |
| 15 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.6 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 16 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 17 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace element solution SL--10 (see Medium [M433])
Dose: 1 G_PER_L
Cross-reference to Medium M433
5% NaHCO3 solution
Dose: 40 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M997_NH-JN4_Medium (CultureMech:010425)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.112647Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M997 |
| 2026-01-28T19:48:18.064304Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:29.910328+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:13.783105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010425 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.759135+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NH-JN4 Medium" to "nh_jn4_medium" for consistent matching |
| 2026-05-16T09:03:22.657659+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.396354+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.276664+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.074831+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 45475c986f63bef10aea6c85003991c489d12dec772d8609b48c74a78ae491a2 |