Overview
- External ontology
- TOGO:M999 — Alginate Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M999 Original source: JCM - JCM_M952 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=952
Composition
flowchart LR
CultureMech_010427["alginate_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010427 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010427 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010427 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_K2HPO4["K2HPO4"]
CultureMech_010427 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_010427 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Na2HPO4_2H2O["Na2HPO4・2H2O"]
CultureMech_010427 --> ing5_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_2H2O -.-> CHEBI_34683
ing6_Sodium_alginate["Sodium alginate"]
CultureMech_010427 --> ing6_Sodium_alginate
CHEBI_53311["CHEBI:53311"]
ing6_Sodium_alginate -.-> CHEBI_53311
ing7_HCl["HCl"]
CultureMech_010427 --> ing7_HCl
CHEBI_17883["CHEBI:17883"]
ing7_HCl -.-> CHEBI_17883
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Na2HPO4・2H2O
HO4P.2Na 141.958 g/mol
|
0.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Sodium alginate
(C6H7O6)n.H2NaO 216.121 g/mol
|
10 G_PER_L |
CHEBI:53311
sodium alginate
|
Role: Solidifying component; Properties: Simple component, Organic compound, Defined component |
| 8 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M999_Alginate_Medium (CultureMech:010427)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.116531Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M999 |
| 2026-01-28T19:48:14.813098Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-13T06:20:13.796948Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010427 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.773665+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alginate Medium" to "alginate_medium" for consistent matching |
| 2026-05-16T09:03:22.674258+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:46.413806+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.504360+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.285214+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.096189+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bdeda6fda4dc796b5c84e30f8dd4fc8a30bb946d93aa7a75f9602eee4754dca1 |