Overview
- External ontology
- jcm.grmd:1354 (source link unavailable) — RFODOSPIRILLACEAE MEDIUM (MODIFIED)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1354
Composition
flowchart LR
CultureMech_015837["rfodospirillaceae_medium_modified"]
ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015837 --> ing0_Yeast_extract__BD_Difco_
ing1_Sodium_succinate["Sodium succinate"]
CultureMech_015837 --> ing1_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing1_Sodium_succinate -.-> CHEBI_63675
ing2_Ammonium_acetate["Ammonium acetate"]
CultureMech_015837 --> ing2_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing2_Ammonium_acetate -.-> CHEBI_62947
ing3_0_1__Fe_III__citrate_solution["0.1% Fe(III) citrate solution"]
CultureMech_015837 --> ing3_0_1__Fe_III__citrate_solution
ing4_KH2PO4["KH2PO4"]
CultureMech_015837 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015837 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_015837 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_NH4Cl["NH4Cl"]
CultureMech_015837 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015837 --> ing8_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_2H2O -.-> CHEBI_86158
ing9_0_01__Vitamin_B12_solution["0.01% Vitamin B12 solution"]
CultureMech_015837 --> ing9_0_01__Vitamin_B12_solution
ing10_SL_6_Trace_element_solution__see_Medium_No__167__["SL-6 Trace element solution (see Medium No. 167 )"]
CultureMech_015837 --> ing10_SL_6_Trace_element_solution__see_Medium_No__167__
ing11_L_Cysteine_HCl_H2O["L-Cysteine·HCl·H2O"]
CultureMech_015837 --> ing11_L_Cysteine_HCl_H2O
ing12_Resazurin["Resazurin"]
CultureMech_015837 --> ing12_Resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Resazurin -.-> CHEBI_8806
ing13_Distilled_water["Distilled water"]
CultureMech_015837 --> ing13_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing13_Distilled_water -.-> CHEBI_15377
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract (BD-Difco) | 0.3 G_PER_L | ||
| 2 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1.0 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 3 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
0.5 G_PER_L |
CHEBI:62947
ammonium acetate
|
|
| 4 | 0.1% Fe(III) citrate solution | 5.0 ML_PER_L | ||
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 | 0.01% Vitamin B12 solution | 0.4 ML_PER_L | ||
| 11 | SL-6 Trace element solution (see Medium No. 167 ) | 1.0 ML_PER_L | ||
| 12 | L-Cysteine·HCl·H2O | 0.3 G_PER_L | ||
| 13 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 14 |
Distilled water
H2O 18.015 g/mol
|
1.0 ML_PER_L |
CHEBI:15377
water
|
Preparation
- Mix components thoroughly and adjust pH 6.8.
- Bring to a boil, cool down under a stream of N2, distribute the medium into culture vessels (e.g., 10 ml medium in Hungate type tubes) under N2, seal with butyl rubber stoppers and autoclave.
- After inoculation, cultivate in the light using a tungsten lamp.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1354_RFODOSPIRILLACEAE_MEDIUM_MODIFIED (CultureMech:015837)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:08:45.577503+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1354. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.517682Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-08-06T03:04:35.434007+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 46bffaa5136c3fe0edb2da83de2759e0dfce0a542080bc762957f15d3d0ce82c |