gbs_salts_medium_for_hydrogenobacter

CultureMech:015844 bacterial DEFINED LIQUID
Original name: GBS SALTS MEDIUM FOR HYDROGENOBACTER

Overview

External ontology
jcm.grmd:1366 (source link unavailable) — GBS SALTS MEDIUM FOR HYDROGENOBACTER
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1366

Composition

flowchart LR
    CultureMech_015844["gbs_salts_medium_for_hydrogenobacter"]
    ing0_NaCl["NaCl"]
    CultureMech_015844 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_015844 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Na2SO4["Na2SO4"]
    CultureMech_015844 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015844 --> ing3_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_7H2O -.-> CHEBI_31795
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015844 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_015844 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Mineral_solution_A__see_Medium_No__1353__["Mineral solution A (see Medium No. 1353 )"]
    CultureMech_015844 --> ing6_Mineral_solution_A__see_Medium_No__1353__
    ing7_Distilled_water["Distilled water"]
    CultureMech_015844 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015844 --> ing8_8__NaHCO3_solution_
    ing9_1_0_M_Na2S2O3_solution["1.0 M Na2S2O3 solution"]
    CultureMech_015844 --> ing9_1_0_M_Na2S2O3_solution
    ing10_0_5_M_Na2HPO4_solution__pH_8_0_["0.5 M Na2HPO4 solution (pH 8.0)"]
    CultureMech_015844 --> ing10_0_5_M_Na2HPO4_solution__pH_8_0_

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
3.0 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.15 G_PER_L CHEBI:32588
potassium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
0.3 G_PER_L CHEBI:32149
sodium sulfate
4 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.123 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
14.5 MG_PER_L CHEBI:86158
calcium chloride dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.107 G_PER_L CHEBI:31206
ammonium chloride
7 Mineral solution A (see Medium No. 1353 ) 5.0 ML_PER_L
8 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
9 8% NaHCO3 solution* 12.5 ML_PER_L
10 1.0 M Na2S2O3 solution 2.5 ML_PER_L
11 0.5 M Na2HPO4 solution (pH 8.0) 20.0 ML_PER_L

Preparation

  1. Mix components thoroughly and distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave.
  2. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically): After inoculation, add air to the gas phase (30-40% by volume) and pressurize to 100 KPa with H2-CO2 (4:1, v/v) gas mixture.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-02T07:08:52.945503+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1366. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.403877Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-06T03:04:35.488691+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6e3b40549b62443108709cdcd7f82f12d1b4c96f79ec9674a619d06517f8f2ba