ia91_medium

Overview

External ontology

jcm.grmd:1389 (source link unavailable) — IA91 MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1389

Composition

flowchart LR
    CultureMech_015849["ia91_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015849 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015849 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015849 --> ing2_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_6H2O -.-> CHEBI_86345
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015849 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_015849 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015849 --> ing5_FeCl2_solution__see_Medium_No__187__
    ing6_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015849 --> ing6_Trace_element_solution__see_Medium_No__187__
    ing7_Selenite_tungstate_solution__see_Medium_No__431__["Selenite-tungstate solution (see Medium No. 431 )"]
    CultureMech_015849 --> ing7_Selenite_tungstate_solution__see_Medium_No__431__
    ing8_NaHCO3["NaHCO3"]
    CultureMech_015849 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Resazurin["Resazurin"]
    CultureMech_015849 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Distilled_water["Distilled water"]
    CultureMech_015849 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377
    ing11_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015849 --> ing11_Trace_vitamins___see_Medium_No__197__
    ing12_Culture_supernatant__see_below_["Culture supernatant (see below)"]
    CultureMech_015849 --> ing12_Culture_supernatant__see_below_
    ing13_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015849 --> ing13_5__L_Cysteine_HCl_H2O_solution
    ing14_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015849 --> ing14_5__Na2S_9H2O_solution

Ingredients (15)

Preparation

1. Mix components, except NaHCO3, and adjust pH 7.0.
2. Bring to a boil for 5-10 seconds, then, cool down under a stream of N2-CO2 (80:20, v/v), add NaHCO3, and autoclave under the same gas mixture.
3. After cooling. aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized, and stored under a N2 atmosphere): Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers.
4. Finally, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): Culture supernatant: Centrifuge a grown culture of strain Acc8 (=JCM 39386) in JCM Medium No.
5. 1388 (e.g., 10,000 xg, 10 min.), then, remove and autoclave the supernatant under a N2-CO2 (80:20, v/v) gas mixture.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1389_IA91_MEDIUM (CultureMech:015849)