pfennig_thiofaba_medium

CultureMech:015851 bacterial DEFINED LIQUID
Original name: PFENNIG THIOFABA MEDIUM

Overview

External ontology
jcm.grmd:1393 (source link unavailable) — PFENNIG THIOFABA MEDIUM
Applications
  • Microbial cultivation
pH
4.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1393

Composition

flowchart LR
    CultureMech_015851["pfennig_thiofaba_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015851 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015851 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_015851 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015851 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015851 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_Na2SO4["Na2SO4"]
    CultureMech_015851 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Na2S2O3_5H2O["Na2S2O3·5H2O"]
    CultureMech_015851 --> ing6_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing6_Na2S2O3_5H2O -.-> CHEBI_32150
    ing7_Trace_element_solution__see_Medium_No__899__["Trace element solution (see Medium No. 899 )"]
    CultureMech_015851 --> ing7_Trace_element_solution__see_Medium_No__899__
    ing8_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015851 --> ing8_Trace_vitamins__see_Medium_No__197__
    ing9_Distilled_water["Distilled water"]
    CultureMech_015851 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377
    ing10_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015851 --> ing10_5__Na2S_9H2O_solution

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.33 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.33 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.33 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.33 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Na2SO4
2Na.O4S 142.043 g/mol
2.0 G_PER_L CHEBI:32149
sodium sulfate
7 Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
2.0 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
8 Trace element solution (see Medium No. 899 ) 1.0 ML_PER_L
9 Trace vitamins (see Medium No. 197 ) 10.0 ML_PER_L
10 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
11 5% Na2S·9H2O solution 12.0 ML_PER_L

Preparation

  1. Mix components thoroughly and adjust pH to 4.5.
  2. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles), seal with butyl rubber stoppers and autoclave.
  3. Replace the gas phase with O2-CO2 (10:90, v/v, 50 KPa overpressured), and add per liter the following solution (autoclaved and stored under a N2 gas atmosphere): Readjust pH to 4.3-4.5 if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-02T07:09:00.926422+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1393. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.343938Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-06T03:04:35.554768+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f8893cbf29b5c46abaacdf0e73b9fb8642099a467aca0b4bcba0f33a14e80fbd