Overview
- External ontology
- jcm.grmd:1404 (source link unavailable) — MINERAL CARBONATE MEDIUM WITH CELLOBIOSE
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1404
Composition
flowchart LR
CultureMech_015854["mineral_carbonate_medium_with_cellobiose"]
ing0_Na2CO3["Na2CO3"]
CultureMech_015854 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_015854 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_015854 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_015854 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Yeast_extract["Yeast extract"]
CultureMech_015854 --> ing4_Yeast_extract
ing5_1_M_MgSO4_solution["1 M MgSO4 solution"]
CultureMech_015854 --> ing5_1_M_MgSO4_solution
ing6_1_M_NH4Cl_solution["1 M NH4Cl solution"]
CultureMech_015854 --> ing6_1_M_NH4Cl_solution
ing7_Trace_element_solution__see_Medium_No__1079__["Trace element solution (see Medium No. 1079 )"]
CultureMech_015854 --> ing7_Trace_element_solution__see_Medium_No__1079__
ing8_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
CultureMech_015854 --> ing8_Trace_vitamins___see_Medium_No__197__
ing9_10__Cellobiose_solution_["10% Cellobiose solution*"]
CultureMech_015854 --> ing9_10__Cellobiose_solution_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10.0 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
33.0 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
6.0 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 | Yeast extract | 0.2 G_PER_L | ||
| 6 | 1 M MgSO4 solution | 1.0 ML_PER_L | ||
| 7 | 1 M NH4Cl solution | 4.0 ML_PER_L | ||
| 8 | Trace element solution (see Medium No. 1079 ) | 1.0 ML_PER_L | ||
| 9 | Trace vitamins* (see Medium No. 197 ) | 1.0 ML_PER_L | ||
| 10 | 10% Cellobiose solution* | 10.0 ML_PER_L |
Preparation
- Add components to distilled water and bring volume to 1.0 L.
- Adjust pH to 9.5-9.6, if necessary.
- Autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1404_MINERAL_CARBONATE_MEDIUM_WITH_CELLOBIOSE (CultureMech:015854)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:04.106266+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1404. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.541943Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-08-06T03:04:35.577451+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint de119c0719681cbf4d486492767fd7d01679fb29fdd9a0ac7bb519726dd0da07 |