halothiobacillus_ls2_medium

CultureMech:015859 bacterial DEFINED LIQUID
Original name: HALOTHIOBACILLUS LS2 MEDIUM

Overview

External ontology
jcm.grmd:1419 (source link unavailable) — HALOTHIOBACILLUS LS2 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1419

Composition

flowchart LR
    CultureMech_015859["halothiobacillus_ls2_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_015859 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaCl["NaCl"]
    CultureMech_015859 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015859 --> ing2_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_6H2O -.-> CHEBI_86345
    ing3_Modified_A5_solution__see_below_["Modified A5 solution (see below)"]
    CultureMech_015859 --> ing3_Modified_A5_solution__see_below_
    ing4_Distilled_water["Distilled water"]
    CultureMech_015859 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_8__NaHCO3_solution["8% NaHCO3 solution"]
    CultureMech_015859 --> ing5_8__NaHCO3_solution
    ing6_0_1_M_M_FeCl2_solution["0.1 M M FeCl2 solution"]
    CultureMech_015859 --> ing6_0_1_M_M_FeCl2_solution
    ing7_1_0_M_NH4Cl_solution["1.0 M NH4Cl solution"]
    CultureMech_015859 --> ing7_1_0_M_NH4Cl_solution
    ing8_1_0_M_Sodium_thiosulfate_solution["1.0 M Sodium thiosulfate solution"]
    CultureMech_015859 --> ing8_1_0_M_Sodium_thiosulfate_solution
    ing9_H3BO3["H3BO3"]
    CultureMech_015859 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_015859 --> ing10_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_7H2O -.-> CHEBI_32312
    ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_015859 --> ing11_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_2H2O -.-> CHEBI_75213
    ing12_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_015859 --> ing12_MnCl2_4H2O
    ing13_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_015859 --> ing13_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_5H2O -.-> CHEBI_31440
    ing14_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_015859 --> ing14_CoCl2_6H2O

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NaCl
Cl.Na 58.443 g/mol
1.0 G_PER_L CHEBI:26710
sodium chloride
3 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.12 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 Modified A5 solution (see below) 1.0 ML_PER_L
5 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
6 8% NaHCO3 solution 2.5 ML_PER_L
7 0.1 M M FeCl2 solution 0.4 ML_PER_L
8 1.0 M NH4Cl solution 1.0 ML_PER_L
9 1.0 M Sodium thiosulfate solution 10.0 ML_PER_L
10 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
11 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
0.222 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 MnCl2·4H2O 1.86 G_PER_L
14 CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
0.079 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
15 CoCl2·6H2O 0.04 G_PER_L

Preparation

  1. Mix components, adjust pH to 6.5-7.0, and autoclave.
  2. For preparation of solid media, add 15.0 g/L agar and 0.1 g/L Na2SO4 before autoclaving.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-02T07:09:09.442757+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1419. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.490469Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-06T03:04:35.608943+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c71068f0022b8e70114a020aa7b66fd36c9ebb687c44f56b150c77dfed9a1b58
2026-08-15T20:09:25.764056+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 1 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Distilled water.