methanobrevibacter_cuticularis_medium

Overview

External ontology

jcm.grmd:1424 (source link unavailable) — METHANOBREVIBACTER CUTICULARIS MEDIUM

Applications

• Microbial cultivation

pH

7.7

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1424

Composition

flowchart LR
    CultureMech_015861["methanobrevibacter_cuticularis_medium"]
    ing0_Rumen_fluid__clarified__see_Medium_No__266_["Rumen fluid, clarified (see Medium No. 266)"]
    CultureMech_015861 --> ing0_Rumen_fluid__clarified__see_Medium_No__266_
    ing1_NaCl["NaCl"]
    CultureMech_015861 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KCl["KCl"]
    CultureMech_015861 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015861 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015861 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_015861 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_KH2PO4["KH2PO4"]
    CultureMech_015861 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_Na2SO4["Na2SO4"]
    CultureMech_015861 --> ing7_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing7_Na2SO4 -.-> CHEBI_32149
    ing8_Casamino_acids__BD_Difco_["Casamino acids (BD Difco)"]
    CultureMech_015861 --> ing8_Casamino_acids__BD_Difco_
    ing9_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_015861 --> ing9_Yeast_extract__Oxoid_
    ing10_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015861 --> ing10_FeCl2_solution__see_Medium_No__187__
    ing11_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015861 --> ing11_Trace_element_solution__see_Medium_No__187__
    ing12_Selenite_tungstate_solution__see_Medium_No__431__["Selenite-tungstate solution (see Medium No. 431 )"]
    CultureMech_015861 --> ing12_Selenite_tungstate_solution__see_Medium_No__431__
    ing13_Resazurin["Resazurin"]
    CultureMech_015861 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Distilled_water["Distilled water"]
    CultureMech_015861 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377
    ing15_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015861 --> ing15_8__NaHCO3_solution_
    ing16_1_0_M_HEPES_solution__pH_7_7_["1.0 M HEPES solution (pH 7.7)"]
    CultureMech_015861 --> ing16_1_0_M_HEPES_solution__pH_7_7_
    ing17_Vitamin_solution__see_Medium_No__898__["Vitamin solution (see Medium No. 898 )"]
    CultureMech_015861 --> ing17_Vitamin_solution__see_Medium_No__898__
    ing18_0_1_M_Dithiothreitol_solution["0.1 M Dithiothreitol solution"]
    CultureMech_015861 --> ing18_0_1_M_Dithiothreitol_solution

Ingredients (19)

Preparation

1. Mix components thoroughly, bring to a boil for 5-10 seconds, then, cool down under a stream of H2-CO2 (80:20 v/v).
2. Distribute the medium into suitable culture vessels (e.g., 4.6 ml medium in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers.
3. Autoclave.
4. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): Prior to inoculation, add per liter the following solution (freshly prepared and filtersterilized): Adjust pH 7.7, if necessary.
5. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20v/ v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1424_METHANOBREVIBACTER_CUTICULARIS_MEDIUM (CultureMech:015861)