Overview
- External ontology
- jcm.grmd:1462 (source link unavailable) — METHANOSARCINA T3 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1462
Composition
flowchart LR
CultureMech_015871["methanosarcina_t3_medium"]
ing0_KCl["KCl"]
CultureMech_015871 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_015871 --> ing1_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_6H2O -.-> CHEBI_86345
ing2_NH4Cl["NH4Cl"]
CultureMech_015871 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015871 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_015871 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_NaCl["NaCl"]
CultureMech_015871 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Trace_mineral_solution__see_Medium_No__151__["Trace mineral solution (see Medium No. 151 )"]
CultureMech_015871 --> ing6_Trace_mineral_solution__see_Medium_No__151__
ing7_Ni_Se_W_solution__see_below_["Ni-Se-W solution (see below)"]
CultureMech_015871 --> ing7_Ni_Se_W_solution__see_below_
ing8_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
CultureMech_015871 --> ing8_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing8_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing9_NaHCO3["NaHCO3"]
CultureMech_015871 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Resazurin["Resazurin"]
CultureMech_015871 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Distilled_water["Distilled water"]
CultureMech_015871 --> ing11_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing11_Distilled_water -.-> CHEBI_15377
ing12_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
CultureMech_015871 --> ing12_Trace_vitamins___see_Medium_No__197__
ing13_2_M_trimethylammonium_chloride_solution_["2 M trimethylammonium chloride solution*"]
CultureMech_015871 --> ing13_2_M_trimethylammonium_chloride_solution_
ing14_Methanol_["Methanol*"]
CultureMech_015871 --> ing14_Methanol_
ing15_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
CultureMech_015871 --> ing15_5__L_Cysteine_HCl_H2O_solution
ing16_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
CultureMech_015871 --> ing16_5__Na2S_9H2O_solution
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_015871 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_015871 --> ing18_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_5H2O -.-> CHEBI_131361
ing19_Na2WO2_2H2O["Na2WO2·2H2O"]
CultureMech_015871 --> ing19_Na2WO2_2H2O
ing20_Distilled_water["Distilled water"]
CultureMech_015871 --> ing20_Distilled_water
ing20_Distilled_water -.-> CHEBI_15377
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 2 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.0 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
9.0 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Trace mineral solution (see Medium No. 151 ) | 10.0 ML_PER_L | ||
| 8 | Ni-Se-W solution (see below) | 10.0 ML_PER_L | ||
| 9 |
Fe(NH4)2(SO4)2·6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
2.0 MG_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5.0 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Distilled water
H2O 18.015 g/mol
|
980.0 ML_PER_L |
CHEBI:15377
water
|
|
| 13 | Trace vitamins* (see Medium No. 197 ) | 10.0 ML_PER_L | ||
| 14 | 2 M trimethylammonium chloride solution* | 35.0 ML_PER_L | ||
| 15 | Methanol* | 5.0 ML_PER_L | ||
| 16 | 5% L-Cysteine·HCl·H2O solution | 10.0 ML_PER_L | ||
| 17 | 5% Na2S·9H2O solution | 10.0 ML_PER_L | ||
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
30.0 MG_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 MG_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 20 | Na2WO2·2H2O | 0.4 MG_PER_L | ||
| 21 |
Distilled water
H2O 18.015 g/mol
|
1.0 ML_PER_L |
CHEBI:15377
water
|
Preparation
- Mix components thoroughly and adjust pH to 6.5, then distribute the medium into culture vessels under a N2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers.
- After autoclaving, stand the medium overnight.
- Prior to use, add per liter the following solutions (autoclaved or* filter-sterilized, and stored under a N2 atmosphere): Adjust pH to 7.0 if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1462_METHANOSARCINA_T3_MEDIUM (CultureMech:015871)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:22.793327+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1462. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.562994Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-08-06T03:04:20.591635+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2f4f9afb1940174e36a9fb6e77dc535802ed50811b93085252987526d5b581aa |