modified_nms_medium_4

CultureMech:015872 bacterial DEFINED LIQUID
Original name: MODIFIED NMS MEDIUM-4

Overview

External ontology
jcm.grmd:1465 (source link unavailable) — MODIFIED NMS MEDIUM-4
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1465

Composition

flowchart LR
    CultureMech_015872["modified_nms_medium_4"]
    ing0_KNO3["KNO3"]
    CultureMech_015872 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015872 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015872 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_015872 --> ing3_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing3_Fe_III__EDTA -.-> CHEBI_30729
    ing4_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_015872 --> ing4_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_2H2O -.-> CHEBI_75213
    ing5_Na2HPO4_12H2O["Na2HPO4·12H2O"]
    CultureMech_015872 --> ing5_Na2HPO4_12H2O
    ing6_KH2PO4["KH2PO4"]
    CultureMech_015872 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_Trace_element_solution__see_below_["Trace element solution (see below)"]
    CultureMech_015872 --> ing7_Trace_element_solution__see_below_
    ing8_Distilled_water["Distilled water"]
    CultureMech_015872 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_015872 --> ing9_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_5H2O -.-> CHEBI_31440
    ing10_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_015872 --> ing10_FeSO4_7H2O
    ing11_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_015872 --> ing11_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_7H2O -.-> CHEBI_32312
    ing12_H3BO2["H3BO2"]
    CultureMech_015872 --> ing12_H3BO2
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO2 -.-> CHEBI_33118
    ing13_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_015872 --> ing13_CoCl2_6H2O
    ing14_EDTA_2Na["EDTA·2Na"]
    CultureMech_015872 --> ing14_EDTA_2Na
    ing15_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_015872 --> ing15_MnCl2_4H2O
    ing16_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_015872 --> ing16_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_6H2O -.-> CHEBI_34887

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
1.0 G_PER_L CHEBI:63043
potassium nitrate
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
1.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
3.8 MG_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
5 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.26 MG_PER_L CHEBI:75213
sodium molybdate dihydrate
6 Na2HPO4·12H2O 0.72 G_PER_L
7 KH2PO4
H2O4P.K 136.084 g/mol
0.26 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 Trace element solution (see below) 1.0 ML_PER_L
9 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
10 CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 FeSO4·7H2O 0.5 G_PER_L
12 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
0.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 H3BO2
H3BO3 61.833 g/mol
0.02 G_PER_L CHEBI:33118
boric acid
14 CoCl2·6H2O 0.05 G_PER_L
15 EDTA·2Na 0.25 G_PER_L
16 MnCl2·4H2O 0.02 G_PER_L
17 NiCl2·6H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride

Preparation

  1. Mix components and autoclave.
  2. Aseptically distribute the medium into sterile serum bottles (leave ≥ 80% gas phase by volume) and seal with rubber stoppers.
  3. Replace the gas phase with an air-methane gas mixture (82:18, v/v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-02T07:09:24.407182+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1465. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.425998Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-06T03:04:35.700043+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6e45c497b2aa93620f9f748805537564e6ab77a3b41cf837e2466e4729f4148b
2026-08-15T20:09:32.423267+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 1 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Distilled water.