Overview
- External ontology
- jcm.grmd:1358 (source link unavailable) — MINERAL CARBONATE MEDIUM WITH ALGINATE
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1358
Composition
flowchart LR
CultureMech_015884["mineral_carbonate_medium_with_alginate"]
ing0_Na2CO3["Na2CO3"]
CultureMech_015884 --> ing0_Na2CO3
ing1_NaHCO3["NaHCO3"]
CultureMech_015884 --> ing1_NaHCO3
ing2_NaCl["NaCl"]
CultureMech_015884 --> ing2_NaCl
ing3_K2HPO4["K2HPO4"]
CultureMech_015884 --> ing3_K2HPO4
ing4_Yeast_extract["Yeast extract"]
CultureMech_015884 --> ing4_Yeast_extract
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Na2CO3 | 27.7 G_PER_L | ||
| 2 | NaHCO3 | 30.6 G_PER_L | ||
| 3 | NaCl | 7.5 G_PER_L | ||
| 4 | K2HPO4 | 1.0 G_PER_L | ||
| 5 | Yeast extract | 0.2 G_PER_L |
Preparation
- Add components to distilled water and bring volume to 1.0 L.
- Adjust pH to 9.5-9.6, if necessary.
- Autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1358_MINERAL_CARBONATE_MEDIUM_WITH_ALGINATE (CultureMech:015884)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-01T01:54:22.069586+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1358. Ingredients captured verbatim. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-10-01T02:55:00+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015884 |
| 2026-10-01T03:00:53.930083+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ee09e966661ff5e0651b1b7e59c63f47a9fa7fb1a20042a7aa9c9b57d721025c |