Overview
- External ontology
- jcm.grmd:1452 (source link unavailable) — PYA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1452
Composition
flowchart LR
CultureMech_015887["pya_medium"]
ing0_Peptone["Peptone"]
CultureMech_015887 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_015887 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_015887 --> ing2_K2HPO4
ing3_1_M_NaHCO3_Na2CO3_buffer__pH_10_0_["1 M NaHCO3/Na2CO3 buffer (pH 10.0)"]
CultureMech_015887 --> ing3_1_M_NaHCO3_Na2CO3_buffer__pH_10_0_
ing4_Distilled_water["Distilled water"]
CultureMech_015887 --> ing4_Distilled_water
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 8.0 G_PER_L | ||
| 2 | Yeast extract | 3.0 G_PER_L | ||
| 3 | K2HPO4 | 1.0 G_PER_L | ||
| 4 | 1 M NaHCO3/Na2CO3 buffer (pH 10.0) | 100.0 ML_PER_L | ||
| 5 | Distilled water | 900.0 ML_PER_L |
Preparation
- Separately autoclave 1 M NaHCO3/Na2CO3 buffer (pH 10.0), aseptically add to the medium (final conc.
- 100 mM).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1452_PYA_MEDIUM (CultureMech:015887)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-01T01:54:27.077244+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1452. Ingredients captured verbatim. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-10-01T02:55:00+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015887 |
| 2026-10-01T03:00:53.960173+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9a88beaf8b1baf700bf66f57121caf4e0b2ec151265780d3a14233927e6a54c1 |