Overview
- Applications
-
- Protozoa cultivation
- Notes
- CCAP prepares FCGM by boiling Ward's cereal grass leaves in deionised water, steeping, filtering, and autoclaving.
Composition
flowchart LR
CultureMech_015893["fcgm_freshwater_cereal_grass_medium"]
ing0_Deionised_water["Deionised water"]
CultureMech_015893 --> ing0_Deionised_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionised_water -.-> CHEBI_15377
ing1_Cereal_grass_leaves["Cereal grass leaves"]
CultureMech_015893 --> ing1_Cereal_grass_leaves
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionised water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
CCAP lists 500 mL deionised water per 500 mL medium. |
| 2 | Cereal grass leaves | 1.25 G_PER_L | Ward's cereal grass leaves; CCAP lists 0.625 g per 500 mL medium. |
Preparation
- Bring the deionised water to the boil.
- Add the cereal grass leaves, boil for 5 minutes, then leave to steep for 3 hours.
- Filter through a Buchner funnel.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- fcgm_freshwater_cereal_grass_medium (CultureMech:015893)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-01T02:46:22+00:00 | codex-agent | ADDED_CCAP_MISSING_ALGAE_MEDIUM | Live CCAP index advertised FCGM and the exact CCAP:FCGM source was absent from data/normalized_yaml before this add-only curation pass. |
| 2026-10-01T02:55:00+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015893 |
| 2026-10-01T03:00:54.022671+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 372af23854d8aff8ee94b66a00180b83f3740f2e58de724390a6e4249976a817 |