sag_bg_11_medium

CultureMech:015897 algae DEFINED LIQUID
Original name: BG 11 Medium for Cyanobacteria

Overview

Applications
  • Cyanobacteria cultivation
Notes
SAG prepares BG 11 from mineral stocks plus a Kuhl and Lorenzen micronutrient solution containing boric acid, manganese sulfate monohydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, and ammonium heptamolybdate tetrahydrate.

Composition

flowchart LR
    CultureMech_015897["sag_bg_11_medium"]
    ing0_De_ionized_or_distilled_water["De-ionized or distilled water"]
    CultureMech_015897 --> ing0_De_ionized_or_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_De_ionized_or_distilled_water -.-> CHEBI_15377
    ing1_NaNO3["NaNO3"]
    CultureMech_015897 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_K2HPO4_x_3H2O["K2HPO4 x 3H2O"]
    CultureMech_015897 --> ing2_K2HPO4_x_3H2O
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4_x_3H2O -.-> CHEBI_131527
    ing3_MgSO4_x_7H2O["MgSO4 x 7H2O"]
    CultureMech_015897 --> ing3_MgSO4_x_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2H2O["CaCl2 x 2H2O"]
    CultureMech_015897 --> ing4_CaCl2_x_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2H2O -.-> CHEBI_86158
    ing5_Citric_acid["Citric acid"]
    CultureMech_015897 --> ing5_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing5_Citric_acid -.-> CHEBI_30769
    ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_015897 --> ing6_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing7_EDTA__dinatrium_salt_["EDTA (dinatrium-salt)"]
    CultureMech_015897 --> ing7_EDTA__dinatrium_salt_
    CHEBI_64734["CHEBI:64734"]
    ing7_EDTA__dinatrium_salt_ -.-> CHEBI_64734
    ing8_Na2CO3["Na2CO3"]
    CultureMech_015897 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 De-ionized or distilled water
H2O 18.015 g/mol
919 ML_PER_L CHEBI:15377
water
 SAG lists 919 mL/L water after the eight 10 mL mineral-stock additions and 1 mL micronutrient solution.
2 NaNO3
NO3.Na 84.994 g/mol
1.5 G_PER_L CHEBI:63005
sodium nitrate
 SAG adds 10 mL/L of a 15 g/100 mL stock.
3 K2HPO4 x 3H2O
HO4P.2K 174.174 g/mol
0.04 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 SAG adds 10 mL/L of a 0.4 g/100 mL stock.
4 MgSO4 x 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.075 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 SAG adds 10 mL/L of a 0.75 g/100 mL stock.
5 CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.036 G_PER_L CHEBI:86158
calcium chloride dihydrate
 SAG adds 10 mL/L of a 0.36 g/100 mL stock.
6 Citric acid
C6H8O7 192.123 g/mol
0.006 G_PER_L CHEBI:30769
citric acid
 SAG adds 10 mL/L of a 0.06 g/100 mL stock.
7 Ferric ammonium citrate 0.006 G_PER_L CHEBI:31604
ferric ammonium citrate
 SAG adds 10 mL/L of a 0.06 g/100 mL stock.
8 EDTA (dinatrium-salt)
C10H14N2O8.2Na 336.208 g/mol
0.001 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 SAG adds 10 mL/L of a 0.01 g/100 mL stock.
9 Na2CO3
CO3.2Na 105.988 g/mol
0.02 G_PER_L CHEBI:29377
sodium carbonate
 SAG adds 10 mL/L of a 0.2 g/100 mL stock.

Preparation

  1. Add the NaNO3, K2HPO4 x 3H2O, MgSO4 x 7H2O, CaCl2 x 2H2O, citric acid, ferric ammonium citrate, EDTA, and Na2CO3 stocks to de-ionized or distilled water.
  2. Add 1 mL/L micronutrient solution and bring to the final volume.

Solutions

Micronutrient solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
H3BO3 61.0 MG_PER_L
MnSO4 x H2O 169.0 MG_PER_L
ZnSO4 x 7H2O 287.0 MG_PER_L
CuSO4 x 5H2O 2.5 MG_PER_L
(NH4)6Mo7O24 x 4H2O 12.5 MG_PER_L
SAG lists the Kuhl and Lorenzen micronutrient solution at 1 mL/L in final BG 11.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-10-01T02:46:22+00:00 codex-agent ADDED_SAG_MISSING_ALGAE_MEDIUM Live SAG index advertised BG11 and the exact SAG:BG11 source was absent from data/normalized_yaml before this add-only curation pass.
2026-10-01T02:55:00+00:00 culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:015897
2026-10-01T03:00:54.088328+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 73ad192a038122051f6c664fd7780ccd0c427d3d64e92048863a8c7882f285ed