Overview
- External ontology
- nbrc.medium:287 (source link unavailable) — NBRC Medium 287
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=287
Composition
flowchart LR
CultureMech_015900["nbrc_medium_287"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_015900 --> ing0__NH4_2SO4
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015900 --> ing1_CaCl2_2H2O
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015900 --> ing2_MgSO4_7H2O
ing3_KH2PO4["KH2PO4"]
CultureMech_015900 --> ing3_KH2PO4
ing4_Na2SiO3_9H2O["Na2SiO3·9H2O"]
CultureMech_015900 --> ing4_Na2SiO3_9H2O
ing5_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_015900 --> ing5_Na2MoO4_2H2O
ing6_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_015900 --> ing6_FeSO4_7H2O
ing7_Yeast_extract["Yeast extract"]
CultureMech_015900 --> ing7_Yeast_extract
ing8_Sulfur__powder_["Sulfur (powder)"]
CultureMech_015900 --> ing8_Sulfur__powder_
CHEBI_26833["CHEBI:26833"]
ing8_Sulfur__powder_ -.-> CHEBI_26833
ing9_Distilled_water["Distilled water"]
CultureMech_015900 --> ing9_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing9_Distilled_water -.-> CHEBI_15377
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | (NH4)2SO4 | 1.3 G_PER_L | ||
| 2 | CaCl2·2H2O | 0.08 G_PER_L | ||
| 3 | MgSO4·7H2O | 0.25 G_PER_L | ||
| 4 | KH2PO4 | 0.28 G_PER_L | ||
| 5 | Na2SiO3·9H2O | 0.1 G_PER_L | ||
| 6 | Na2MoO4·2H2O | 0.03 G_PER_L | ||
| 7 | FeSO4·7H2O | 0.02 G_PER_L | ||
| 8 | Yeast extract | 1 G_PER_L | ||
| 9 |
Sulfur (powder)
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 10 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 2.5 with 6 N H2SO4
- *Yeast extract is autoclaved separately as 10% solution.
- **Sulfur is sterilized separately by autoclaving for 60 min at 105C on each of 3 successive days and added after inoculation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nbrc_medium_287 (CultureMech:015900)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 287. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015900 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e02a51bb42783d953bb9d1c65135fce8bcb8726179bb88f2a33a2f8a204cd1b2 |