Overview
- External ontology
- nbrc.medium:355 (source link unavailable) — R-CW medium
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=355
Composition
flowchart LR
CultureMech_015901["r_cw_medium_nbrc_355"]
ing0_Peptone["Peptone"]
CultureMech_015901 --> ing0_Peptone
ing1_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_015901 --> ing1_Bacto_Tryptone__Difco_
ing2_Yeast_extract["Yeast extract"]
CultureMech_015901 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_015901 --> ing3_KH2PO4
ing4_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
CultureMech_015901 --> ing4_Sodium_acetate_trihydrate
ing5_Diammonium_hydrogencitrate["Diammonium hydrogencitrate"]
CultureMech_015901 --> ing5_Diammonium_hydrogencitrate
ing6_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015901 --> ing6_MgSO4_7H2O
ing7_MnSO4_nH2O["MnSO4·nH2O"]
CultureMech_015901 --> ing7_MnSO4_nH2O
ing8_Tween_80["Tween 80"]
CultureMech_015901 --> ing8_Tween_80
CHEBI_53426["CHEBI:53426"]
ing8_Tween_80 -.-> CHEBI_53426
ing9_Cheese_whey["Cheese whey"]
CultureMech_015901 --> ing9_Cheese_whey
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Bacto Tryptone (Difco) | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 | KH2PO4 | 5 G_PER_L | ||
| 5 | Sodium acetate trihydrate | 5 G_PER_L | ||
| 6 | Diammonium hydrogencitrate | 2 G_PER_L | ||
| 7 | MgSO4·7H2O | 0.5 G_PER_L | ||
| 8 | MnSO4·nH2O | 0.5 G_PER_L | ||
| 9 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 ML_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 10 | Cheese whey | 1 L |
Preparation
- * Add HCl to warmed milk and coagulate. After centrifuging, use the supernatant.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- r_cw_medium_nbrc_355 (CultureMech:015901)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 355. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015901 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a959a8225c24307371d73a379b83be012e90dfd40b575239a28ea15a0faa9d75 |