Overview
- External ontology
- nbrc.medium:943 (source link unavailable) — Modified Prosthecomicrobium and Ancalomicrobium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=943
Composition
flowchart LR
CultureMech_015904["modified_prosthecomicrobium_and_ancalomicrobium_medium_nbrc_943"]
ing0_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_015904 --> ing0_Bacto_Peptone__Difco_
ing1_Yeast_extract["Yeast extract"]
CultureMech_015904 --> ing1_Yeast_extract
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_015904 --> ing2__NH4_2SO4
ing3_Glucose["Glucose"]
CultureMech_015904 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Vitamin_solution["Vitamin solution"]
CultureMech_015904 --> ing4_Vitamin_solution
ing5_Modified_Hutner_s_solution["Modified Hutner's solution"]
CultureMech_015904 --> ing5_Modified_Hutner_s_solution
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_015904 --> ing6_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing6_Agar__if_needed_ -.-> CHEBI_2509
ing7_Distilled_water["Distilled water"]
CultureMech_015904 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Peptone (Difco) | 0.15 G_PER_L | ||
| 2 | Yeast extract | 0.15 G_PER_L | ||
| 3 | (NH4)2SO4 | 0.25 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Vitamin solution | 10 ML_PER_L | ||
| 6 | Modified Hutner's solution | 20 ML_PER_L | ||
| 7 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
NBRC lists this component as optional when a solid medium is needed. |
| 8 |
Distilled water
H2O 18.015 g/mol
|
970 ML_PER_L |
CHEBI:15377
water
|
Preparation
- pH unadjusted
- Adjust volume to 1 L with distilled water.
- Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6-6.8 before bringing the volume to 1.0 L with distilled water.
- Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store in the dark and cold (5C).
Solutions
Vitamin solution
Dose: 10 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2 MG_PER_L | |
| Folic acid | 2 MG_PER_L | |
| Thiamine-HCl | 5 MG_PER_L | |
| Ca-pantothenate | 5 MG_PER_L | |
| Vitamin B12 | 0.1 MG_PER_L | |
| Riboflavin | 5 MG_PER_L | |
| Distilled water | 1 L |
Modified Hutner's solution
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid (NTA) | 10 G_PER_L | |
| MgSO4·7H2O | 29.7 G_PER_L | |
| CaCl2·2H2O | 3.34 G_PER_L | |
| (NH4)6Mo7O24·2H2O | 9.25 MG_PER_L | |
| FeSO4·7H2O | 99 MG_PER_L | |
| Metal salt solution 44 | 50 ML_PER_L |
Metal salt solution 44
Dose: 50 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na-EDTA | 250 MG_PER_L | |
| ZnSO4·7H2O | 1095 MG_PER_L | |
| FeSO4·7H2O | 0.5 G_PER_L | |
| MnSO4·H2O | 154 MG_PER_L | |
| CuSO4·5H2O | 39.2 MG_PER_L | |
| Co(NO3)2·6H2O | 24.8 MG_PER_L | |
| Na2B4O7·10H2O | 17.7 MG_PER_L | |
| Distilled water | 100 ML_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_prosthecomicrobium_and_ancalomicrobium_medium_nbrc_943 (CultureMech:015904)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 943. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015904 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0f6e18cf16a692c8227a90c340a8e67dcee6183326c3f14321675e19714b6d58 |