Overview
- External ontology
- nbrc.medium:944 (source link unavailable) — Peptone Yeast Extract Alkaline Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=944
Composition
flowchart LR
CultureMech_015905["peptone_yeast_extract_alkaline_medium_nbrc_944"]
ing0_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_015905 --> ing0_Bacto_Peptone__Difco_
ing1_Yeast_extract["Yeast extract"]
CultureMech_015905 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_015905 --> ing2_K2HPO4
ing3_EDTA_2Na["EDTA-2Na"]
CultureMech_015905 --> ing3_EDTA_2Na
ing4_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_015905 --> ing4_ZnSO4_7H2O
ing5_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_015905 --> ing5_FeSO4_7H2O
ing6_MnSO4_nH2O["MnSO4·nH2O"]
CultureMech_015905 --> ing6_MnSO4_nH2O
ing7_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_015905 --> ing7_CuSO4_5H2O
ing8_Co_NO3_2_6H2O["Co(NO3)2·6H2O"]
CultureMech_015905 --> ing8_Co_NO3_2_6H2O
ing9_H3BO3["H3BO3"]
CultureMech_015905 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_Agar__if_needed_["Agar (if needed)"]
CultureMech_015905 --> ing10_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing10_Agar__if_needed_ -.-> CHEBI_2509
ing11_100_mM_NaHCO3_100_mM_Na2CO3_buffer__pH_10_["100 mM NaHCO3/100 mM Na2CO3 buffer (pH 10)"]
CultureMech_015905 --> ing11_100_mM_NaHCO3_100_mM_Na2CO3_buffer__pH_10_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Peptone (Difco) | 8 G_PER_L | ||
| 2 | Yeast extract | 3 G_PER_L | ||
| 3 | K2HPO4 | 1 G_PER_L | ||
| 4 | EDTA-2Na | 3.5 MG_PER_L | ||
| 5 | ZnSO4·7H2O | 3 MG_PER_L | ||
| 6 | FeSO4·7H2O | 10 MG_PER_L | ||
| 7 | MnSO4·nH2O | 2 MG_PER_L | ||
| 8 | CuSO4·5H2O | 1 MG_PER_L | ||
| 9 | Co(NO3)2·6H2O | 2 MG_PER_L | ||
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
1 MG_PER_L |
CHEBI:33118
boric acid
|
|
| 11 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
NBRC lists this component as optional when a solid medium is needed. |
| 12 | 100 mM NaHCO3/100 mM Na2CO3 buffer (pH 10) | 1 L |
Preparation
- pH unadjusted
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- peptone_yeast_extract_alkaline_medium_nbrc_944 (CultureMech:015905)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 944. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015905 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6ae714006518375cfa45dc62977e7b9b29ee72d462f061db40300100e5682f3e |