Overview
- External ontology
- nbrc.medium:1096 (source link unavailable) — 1/5 Marine Agar with Phenanthrene
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1096
Composition
flowchart LR
CultureMech_015906["one_fifth_marine_agar_with_phenanthrene_nbrc_1096"]
ing0_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
CultureMech_015906 --> ing0_Bacto_Marine_Broth_2216__Difco_
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_015906 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_015906 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
ing3_Sea_water["Sea water"]
CultureMech_015906 --> ing3_Sea_water
ing4_Distilled_water["Distilled water"]
CultureMech_015906 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_Phenanthrene["Phenanthrene"]
CultureMech_015906 --> ing5_Phenanthrene
ing6_Ethanol["Ethanol"]
CultureMech_015906 --> ing6_Ethanol
CHEBI_16236["CHEBI:16236"]
ing6_Ethanol -.-> CHEBI_16236
ing7_1_5_Marine_Agar["1/5 Marine Agar"]
CultureMech_015906 --> ing7_1_5_Marine_Agar
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Marine Broth 2216 (Difco) | 7.5 G_PER_L | ||
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 3 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
NBRC lists this component as optional when a solid medium is needed. |
| 4 | Sea water | 800 ML_PER_L | ||
| 5 |
Distilled water
H2O 18.015 g/mol
|
200 ML_PER_L |
CHEBI:15377
water
|
|
| 6 | Phenanthrene | 51 MG_PER_L | ||
| 7 |
Ethanol
C2H6O 46.069 g/mol
|
6 ML_PER_L |
CHEBI:16236
ethanol
|
|
| 8 | 1/5 Marine Agar | 100 ML_PER_L |
Preparation
- Layered the Phenanthrene-Agar*** on the 1/5 Marine Agar plate.
- *Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)
- **Wako Pure Chemical Ind., Ltd., Osaka, Japan.
- ***Phenanthrene-Agar
- Sterilize separately by filtration.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- one_fifth_marine_agar_with_phenanthrene_nbrc_1096 (CultureMech:015906)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 1096. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015906 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 537557c11ad5b00d9f9f418e221454272e45391a630c124dce7f66b71913e7e4 |