glycerol_fermentation_medium_for_diet_coculture

CultureMech:015432 specialized DEFINED LIQUID
Original name: Glycerol Fermentation Medium for DIET Coculture

Overview

pH
6.9
Notes
Headspace gas: N2 (>99.995%) Vessel type: 100 mL serum bottles with 50 mL working volume Inoculum source: C. pasteurianum full grown pre-culture (1/50 dilution) and concentrated G. sulfurreducens suspension Medium supplemented with 10 mL vitamin solution (DSMZ medium 141), 10 mL trace element solution (DSMZ medium 141), and 0.5 mL trace element solution SL-10 (DSMZ medium 320) per liter. Cultures maintained at 35°C with constant agitation under strict anaerobic conditions using Hungate technique. Physical contact between bacteria enables electron exchange either directly via interspecies wiring or indirectly using soluble electron mediators like L-cysteine present in medium.
Cross-Mech
MediaIngredientMech · CommunityMech · TaxonMech · TraitMech · HabitatMech · CMMMech

Composition

flowchart LR
    CultureMech_015432["glycerol_fermentation_medium_for_diet_coculture"]
    ing0_Glycerol["Glycerol"]
    CultureMech_015432 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_Sodium_acetate["Sodium acetate"]
    CultureMech_015432 --> ing1_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Sodium_acetate -.-> CHEBI_32954
    ing2_Ammonium_chloride["Ammonium chloride"]
    CultureMech_015432 --> ing2_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing2_Ammonium_chloride -.-> CHEBI_31206
    ing3_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_015432 --> ing3_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing3_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing4_Disodium_hydrogen_phosphate["Disodium hydrogen phosphate"]
    CultureMech_015432 --> ing4_Disodium_hydrogen_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing4_Disodium_hydrogen_phosphate -.-> CHEBI_34683
    ing5_Sodium_sulfate["Sodium sulfate"]
    CultureMech_015432 --> ing5_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing5_Sodium_sulfate -.-> CHEBI_32149
    ing6_Magnesium_chloride_hexahydrate["Magnesium chloride hexahydrate"]
    CultureMech_015432 --> ing6_Magnesium_chloride_hexahydrate
    CHEBI_86345["CHEBI:86345"]
    ing6_Magnesium_chloride_hexahydrate -.-> CHEBI_86345
    ing7_Calcium_chloride_dihydrate["Calcium chloride dihydrate"]
    CultureMech_015432 --> ing7_Calcium_chloride_dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing7_Calcium_chloride_dihydrate -.-> CHEBI_86158
    ing8_L_Cysteine["L-Cysteine"]
    CultureMech_015432 --> ing8_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing8_L_Cysteine -.-> CHEBI_17561

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 10.0 G_PER_L CHEBI:17754
glycerol
2 Sodium acetate
C2H3O2.Na 82.034 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.82 G_PER_L CHEBI:32954
sodium acetate
3 Ammonium chloride
Cl.H4N 53.492 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 2.0 G_PER_L CHEBI:31206
ammonium chloride
4 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 2.45 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
5 Disodium hydrogen phosphate
HO4P.2Na 141.958 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 4.58 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 Sodium sulfate
2Na.O4S 142.043 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.28 G_PER_L CHEBI:32149
sodium sulfate
7 Magnesium chloride hexahydrate
2Cl.6H2O.Mg 203.301 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.26 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Calcium chloride dihydrate
Ca.2Cl.2H2O 147.014 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 2.9 MG_PER_L CHEBI:86158
calcium chloride dihydrate
9 L-Cysteine
C3H7NO2S 121.161 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.5 G_PER_L CHEBI:17561
L-cysteine

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-15T00:15:00.375852Z communitymech-importer-v1.0 Imported from CommunityMech Created from CommunityMech:000031 growth_media record
2026-03-17T17:06:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:33.841080+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Glycerol Fermentation Medium for DIET Coculture" to "glycerol_fermentation_medium_for_diet_coculture" for consistent matching
2026-05-24T04:38:33.263218+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=1 env(s) from CommunityMech
2026-06-05T07:41:57.371680+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-09T01:42:26.531150+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-09-13T00:00:00-07:00 repair_communitymech_sources_score10_batch10.py ADDED_COMMUNITYMECH_STRUCTURED_SOURCES_BATCH10 Promoted CommunityMech upstream IDs into sources[] for source-provenance scoring.