half_strength_murashige_skoog_medium_for_arabidopsis_growth

CultureMech:015433 specialized DEFINED LIQUID
Original name: Half-strength Murashige-Skoog medium for Arabidopsis growth

Overview

pH
5.7
Notes
Vessel type: square petri plates (vertical orientation) Light regime: 16h light / 8h dark Light intensity: 120 Gnotobiotic plant growth system with 0.5× MS medium supplemented with 1% sucrose and 0.8% agar. Seeds surface-sterilized prior to inoculation. Plates oriented vertically to allow root observation and bacterial colonization tracking.
Cross-Mech
MediaIngredientMech · CommunityMech · TaxonMech · TraitMech · HabitatMech · CMMMech

Composition

flowchart LR
    CultureMech_015433["half_strength_murashige_skoog_medium_for_arabidopsis_growth"]
    ing0_Murashige_Skoog_basal_salts["Murashige-Skoog basal salts"]
    CultureMech_015433 --> ing0_Murashige_Skoog_basal_salts
    ing1_Sucrose["Sucrose"]
    CultureMech_015433 --> ing1_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing1_Sucrose -.-> CHEBI_17992
    ing2_Agar["Agar"]
    CultureMech_015433 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Murashige-Skoog basal salts
Cross-Mech: MediaIngredientMech · CMMMech
 0.5 VARIABLE
2 Sucrose
C12H22O11 342.297 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 1.0 PERCENT_W_V CHEBI:17992
sucrose
3 Agar
Cross-Mech: MediaIngredientMech · CMMMech
 0.8 PERCENT_W_V CHEBI:2509
agar

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-15T00:15:00.393357Z communitymech-importer-v1.0 Imported from CommunityMech Created from CommunityMech:000003, CommunityMech:000022 growth_media record
2026-03-17T19:46:33.845908+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Half-strength Murashige-Skoog medium for Arabidopsis growth" to "half_strength_murashige_skoog_medium_for_arabidopsis_growth" for consistent matching
2026-05-17T07:07:47.519318+00:00 cleanup_residual_errors.py CLEANUP_RESIDUAL_ERRORS R04_fill_missing_explanation=1
2026-05-24T04:38:33.269053+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=1 env(s) from CommunityMech
2026-06-05T07:41:57.378147+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_communitymech_sources_score10_batch10.py ADDED_COMMUNITYMECH_STRUCTURED_SOURCES_BATCH10 Promoted CommunityMech upstream IDs into sources[] for source-provenance scoring.