modified_dsm_120_medium_for_diet_coculture

Overview

Notes

Headspace gas: N2/CO2 (80:20) Vessel type: Anaerobic serum bottles with thick butyl rubber stoppers Modified DSM 120 medium with no yeast extract, Casitone, or resazurin. Specific composition: 0.5 mM sulfide, 1 mM cysteine, 0.002 g/L CaCl2·2H2O, 1 g/L NaCl, 2 g/L NaHCO3. Medium boiled to reduce oxygen, cooled under N2/CO2 gas mixture. Sulfide and cysteine essential for long-term M. barkeri cultivation. Initial cocultures: 0.5 ml each organism in 9 ml medium; later transfers: 10% inocula in 45 ml medium.

Cross-Mech

MediaIngredientMech · CommunityMech · TaxonMech · TraitMech · HabitatMech · CMMMech

Composition

flowchart LR
    CultureMech_015434["modified_dsm_120_medium_for_diet_coculture"]
    ing0_Ethanol["Ethanol"]
    CultureMech_015434 --> ing0_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing0_Ethanol -.-> CHEBI_16236
    ing1_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_015434 --> ing1_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing1_Sodium_bicarbonate -.-> CHEBI_32139
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_015434 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Calcium_chloride_dihydrate["Calcium chloride dihydrate"]
    CultureMech_015434 --> ing3_Calcium_chloride_dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing3_Calcium_chloride_dihydrate -.-> CHEBI_86158
    ing4_L_Cysteine["L-Cysteine"]
    CultureMech_015434 --> ing4_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing4_L_Cysteine -.-> CHEBI_17561
    ing5_Sodium_sulfide["Sodium sulfide"]
    CultureMech_015434 --> ing5_Sodium_sulfide
    CHEBI_76208["CHEBI:76208"]
    ing5_Sodium_sulfide -.-> CHEBI_76208

Ingredients (6)

Target organisms

No target organisms are recorded.

Provenance