nitrogen_free_medium_for_leptospirillum_ferrodiazotrophum

Overview

pH

1.7

Notes

Nitrogen-free medium developed for selective isolation of Leptospirillum ferrodiazotrophum (Leptospirillum group III), the nitrogen-fixing member of Richmond Mine biofilm. Richmond Mine AMD biofilm sample naturally abundant in group III was homogenized, filtered, and serially diluted into nitrogen-free liquid medium. Culture grew autotrophically to maximum density ~10^6 cells/ml, oxidizing ferrous iron as sole energy source. Ammonium sulfate deliberately omitted to select for diazotrophic organisms capable of nitrogen fixation. This selective isolation strategy was guided by metagenomic data showing a single nif operon on a Leptospirillum group III genome fragment. Axenic culture confirmed by 16S rRNA-ITS clone library analysis. Note: This medium supports L. ferrodiazotrophum but not the complete biofilm community.

Composition

flowchart LR
    CultureMech_015436["nitrogen_free_medium_for_leptospirillum_ferrodiazotrophum"]
    ing0_Iron_II__sulfate["Iron(II) sulfate"]
    CultureMech_015436 --> ing0_Iron_II__sulfate
    CHEBI_75832["CHEBI:75832"]
    ing0_Iron_II__sulfate -.-> CHEBI_75832
    ing1_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_015436 --> ing1_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing1_Magnesium_sulfate -.-> CHEBI_32599
    ing2_Monopotassium_phosphate["Monopotassium phosphate"]
    CultureMech_015436 --> ing2_Monopotassium_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing2_Monopotassium_phosphate -.-> CHEBI_63036

Ingredients (3)

Target organisms

No target organisms are recorded.

Provenance