nitrogen_free_b_d_medium_for_lotus_japonicus_growth

CultureMech:015437 specialized DEFINED LIQUID
Original name: Nitrogen-free B&D medium for Lotus japonicus growth

Overview

pH
6.5
Notes
Vessel type: growth pouches or square plates Light regime: 16h light / 8h dark Light intensity: 100 Modified Broughton and Dilworth nitrogen-free plant nutrient medium for studying symbiotic nitrogen fixation and nodulation. Gnotobiotic system allowing visualization of root colonization and nodule formation by Mesorhizobium strains. Medium supports both nitrogen-fixing symbionts and non-nodulating commensal bacteria.
Cross-Mech
MediaIngredientMech · CommunityMech · TaxonMech · TraitMech · HabitatMech · CMMMech

Composition

flowchart LR
    CultureMech_015437["nitrogen_free_b_d_medium_for_lotus_japonicus_growth"]
    ing0_Calcium_chloride["Calcium chloride"]
    CultureMech_015437 --> ing0_Calcium_chloride
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride -.-> CHEBI_3312
    ing1_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_015437 --> ing1_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing1_Magnesium_sulfate -.-> CHEBI_32599
    ing2_Potassium_phosphate["Potassium phosphate"]
    CultureMech_015437 --> ing2_Potassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_phosphate -.-> CHEBI_131527
    ing3_Iron_EDTA["Iron-EDTA"]
    CultureMech_015437 --> ing3_Iron_EDTA

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Calcium chloride
Ca.2Cl 110.984 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 1.0 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Magnesium sulfate
Mg.O4S 120.368 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.5 MILLIMOLAR CHEBI:32599
magnesium sulfate
3 Potassium phosphate
HO4P.2K 174.174 g/mol
Cross-Mech: MediaIngredientMech · CMMMech
 0.7 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
4 Iron-EDTA
Cross-Mech: MediaIngredientMech · CMMMech
 50.0 MICROMOLAR

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-15T00:15:00.465121Z communitymech-importer-v1.0 Imported from CommunityMech Created from CommunityMech:000040 growth_media record
2026-03-17T19:46:34.323071+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nitrogen-free B&D medium for Lotus japonicus growth" to "nitrogen_free_b_d_medium_for_lotus_japonicus_growth" for consistent matching
2026-05-17T07:07:49.388030+00:00 cleanup_residual_errors.py CLEANUP_RESIDUAL_ERRORS R04_fill_missing_explanation=1
2026-05-24T04:38:33.289545+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=1 env(s) from CommunityMech
2026-06-05T07:41:58.218259+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_communitymech_sources_score10_batch10.py ADDED_COMMUNITYMECH_STRUCTURED_SOURCES_BATCH10 Promoted CommunityMech upstream IDs into sources[] for source-provenance scoring.