pcs_fp_medium_for_thermophilic_cellulose_degradation

CultureMech:015439 bacterial COMPLEX LIQUID
Original name: PCS-FP medium for thermophilic cellulose degradation

Overview

pH
8.0
Notes
Vessel type: test tubes with loose caps PCS basal medium (peptone-cellulose-salt) supplemented with filter paper as cellulose substrate and calcium carbonate buffer. Medium prepared with loose caps to allow oxygen entry while maintaining high humidity. Static (non-shaking) incubation. The aerobic members (Pseudoxanthomonas, Brevibacillus, Bordetella) consume oxygen to create localized anaerobic microenvironments for the cellulolytic Clostridium species. Inoculum volume: 40 microliters per 4 mL medium. Subculture protocol: 40-700 microliters transferred to fresh medium after 6 days.

Composition

flowchart LR
    CultureMech_015439["pcs_fp_medium_for_thermophilic_cellulose_degradation"]
    ing0_Filter_paper__cellulose_substrate_["Filter paper (cellulose substrate)"]
    CultureMech_015439 --> ing0_Filter_paper__cellulose_substrate_
    CHEBI_18246["CHEBI:18246"]
    ing0_Filter_paper__cellulose_substrate_ -.-> CHEBI_18246
    ing1_Calcium_carbonate["Calcium carbonate"]
    CultureMech_015439 --> ing1_Calcium_carbonate
    CHEBI_3311["CHEBI:3311"]
    ing1_Calcium_carbonate -.-> CHEBI_3311
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_015439 --> ing2_Yeast_extract
    ing3_Peptone["Peptone"]
    CultureMech_015439 --> ing3_Peptone
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_015439 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Filter paper (cellulose substrate)
(C6H10O5)n.H2O 180.156 g/mol
10.0 G_PER_L CHEBI:18246
(1->4)-beta-D-glucan
2 Calcium carbonate
CO3.Ca 100.086 g/mol
2.0 G_PER_L CHEBI:3311
calcium carbonate
3 Yeast extract 1.0 G_PER_L
4 Peptone 5.0 G_PER_L
5 Sodium chloride
Cl.Na 58.443 g/mol
5.0 G_PER_L CHEBI:26710
sodium chloride

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-15T00:15:00.492730Z communitymech-importer-v1.0 Imported from CommunityMech Created from CommunityMech:000061 growth_media record
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.629908+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PCS-FP medium for thermophilic cellulose degradation" to "pcs_fp_medium_for_thermophilic_cellulose_degradation" for consistent matching
2026-05-24T04:38:33.299025+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=1 env(s) from CommunityMech
2026-06-05T07:40:29.566713+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_communitymech_sources_score10_batch10.py ADDED_COMMUNITYMECH_STRUCTURED_SOURCES_BATCH10 Promoted CommunityMech upstream IDs into sources[] for source-provenance scoring.