Beijerinck's Solution

CultureMech:015440 bacterial

Overview

Notes
Stock solution for TAP Medium (Tris-Acetate-Phosphate) and related algae culture media. Provides macrominerals (N, Ca, Mg, S) for freshwater algae. Standard formulation used in Chlamydomonas reinhardtii culture. Preparation: Add components to approximately 800 mL distilled water, dissolve completely, then bring to 1 L total volume. Store at room temperature. Stock amounts are NH4Cl 8 g/L, CaCl2.2H2O 1 g/L and MgSO4.7H2O 2 g/L. The source table's millimolar column is explicitly for TAP medium, not the stock. No universal stock addition dose or replacement molarity is inferred.

Stock composition (3)

# Ingredient Concentration Ontology Notes
1 Ammonium chloride
Cl.H4N 53.492 g/mol
8.0 G_PER_L CHEBI:31206
ammonium chloride
2 Calcium chloride dihydrate
Ca.2Cl.2H2O 147.014 g/mol
1.0 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
2.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-03-15T00:00:00Z mediaingredientmech-migrator-v1.0 Created from MediaIngredientMech migration Composition extracted from UTEX Culture Collection protocols
2026-04-04T17:49:50.350339 categorize_unknown_media CATEGORIZED Automated categorization (confidence: 0.11). Weak match (score: 0.13), defaulting to bacterial
2026-05-16T09:02:58.396968+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS references.reference_id->reference=3
2026-06-08T23:17:55.772491+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-10T06:43:40+00:00 codex CURATED_UTEX_MIGRATED_STOCK_CONCENTRATION_EVIDENCE Source-backed evidence and stock-context review. No structured concentrations or identities changed; conflicts stay explicit.