Overview
- Notes
- Trace metal stock solution for Bold Basal Medium (BBM) and related algae culture media. Provides essential micronutrients (Zn, Mn, Mo, Cu, Co) for freshwater algae. Preparation: Add components to approximately 900 mL deionized water in the order specified while stirring continuously. Add H2SO4 FIRST to acidify the solution before adding metals. This prevents precipitation. Bring to 1 L total volume. Storage: Room temperature in darkness. The acidic pH keeps metals in solution. Source discrepancy: the table's separately labeled concentration columns and its stock-solution footnote do not provide a consistent basis for the former micromolar stock summary. The structured amounts retain the explicit preparation amounts per final 1 L; no replacement molarity or working dilution is asserted. The manganese row prints MnCl.4H2O rather than the assigned MnCl2.4H2O; its supplied-form claim remains unresolved.
Stock composition (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sulfuric acid (concentrated)
H2O4S 98.079 g/mol
|
10 ML_PER_L |
CHEBI:26836
sulfuric acid
|
|
| 2 |
Zinc sulfate heptahydrate
7H2O.O4S.Zn 287.558 g/mol
|
8.82 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 3 |
Manganese chloride tetrahydrate
Cl2Mn.4H2O 197.904 g/mol
|
1.44 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 4 |
Molybdenum trioxide
MoO3 143.937 g/mol
|
0.71 G_PER_L |
CHEBI:30627
molybdenum trioxide
|
|
| 5 |
Copper sulfate pentahydrate
Cu.5H2O.O4S 249.684 g/mol
|
1.57 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 6 |
Cobalt nitrate hexahydrate
Co.6H2O.2NO3 291.031 g/mol
|
0.49 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-03-15T00:00:00Z | mediaingredientmech-migrator-v1.0 | Created from MediaIngredientMech migration | Composition extracted from UTEX Culture Collection protocols |
| 2026-04-04T17:49:50.322458 | categorize_unknown_media | CATEGORIZED | Automated categorization (confidence: 0.11). Weak match (score: 0.13), defaulting to bacterial |
| 2026-05-16T09:02:58.401625+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | references.reference_id->reference=4 |
| 2026-06-08T23:17:55.778773+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-10T06:43:40+00:00 | codex | CURATED_UTEX_MIGRATED_STOCK_CONCENTRATION_EVIDENCE | Source-backed evidence and stock-context review. No structured concentrations or identities changed; conflicts stay explicit. |