Overview
- Notes
- DAS Macro Solution for Dasycladales Seawater Medium. DAS = Dasycladales-specific formulation for marine algae. Provides macronutrients (nitrogen and phosphorus) for marine algae culture. Preparation: Combine 10 mL of 0.70 M sodium nitrate stock with 30 mL of 0.02 M disodium hydrogen phosphate heptahydrate stock to make the stated 40 mL composite stock. Autoclave each component stock before addition; add aseptically in the listed order and swirl gently. The 25% and 75% volume fractions refer to these prepared stocks within this 40 mL mixture, not to the pure solutes. Component stock strengths remain on the nested compositions. Receiving-medium context: UTEX expresses the component additions per 3 L of DAS and separately labels the receiving concentrations as 2.3 mM and 0.2 mM. These are not the component stock strengths or a universal working dose for every medium. Storage: Refrigerator temperature; no exact temperature is established by this source.
Stock composition (0)
No ingredients recorded.
Solutions
Sodium nitrate (0.70 M stock)
Dose: 25.0 PERCENT_V_V
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium nitrate | 0.70 MOLAR |
Prepared-stock fraction of the 40 mL DAS Macro Solution; component stock 1. Autoclave before addition.
Disodium phosphate heptahydrate (0.02 M stock)
Dose: 75.0 PERCENT_V_V
| Ingredient | Concentration | Notes |
|---|---|---|
| Disodium hydrogen phosphate heptahydrate | 0.02 MOLAR | Exact heptahydrate supplied as Sigma S9390. The former CHEBI:34683 mapping describes the anhydrous salt; exact hydrate ontology grounding remains unresolved. The packaged MIM stock-label answer is not treated as proof of hydrate equivalence. |
Prepared-stock fraction of the 40 mL DAS Macro Solution; component stock 2. Autoclave before addition.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-03-15T00:00:00Z | mediaingredientmech-migrator-v1.0 | Created from MediaIngredientMech migration | Composition extracted from UTEX Culture Collection protocols |
| 2026-04-04T17:49:50.357735 | categorize_unknown_media | CATEGORIZED | Automated categorization (confidence: 0.11). Weak match (score: 0.13), defaulting to bacterial |
| 2026-05-16T09:02:58.413261+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | references.reference_id->reference=3 |
| 2026-05-17T07:06:06.184928+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R02_convert_exotic_units=2 |
| 2026-10-10T07:40:20.478855+00:00 | codex | CURATED_UTEX_SEVEN_STOCK_AMOUNTS_FORMS_AND_BOUNDARIES | Source-backed amount, supplied-form and preparation-stage review. Numerical mass concentrations retained; unresolved source conflicts explicitly recorded. No taxa-media association added. |