Overview
- Notes
- DAS Vitamin Cocktail for Dasycladales Seawater Medium. Provides essential vitamins for marine algae culture. Preparation: Add Tricine to approximately 85 mL deionized water while stirring. Adjust pH to 8.0; the source does not specify a titrating reagent. Add the remaining components in the listed order, make up to 100 mL, and filter through a 0.45 um Millipore filter. Unresolved preparation conflict: the Vitamin B12 table row says to add after autoclaving, while the directions specify filtration and do not identify an autoclaving step. This record does not resolve that contradiction or invent a heat-treatment sequence. Storage: In darkness at freezer temperature; the source does not specify -20 C. Concentrations are retained as supplied-product g/L from the stated 100 mL preparation. The printed stock and receiving-DAS molarity columns are not independently verified here; the supplied calcium pantothenate must not be treated as free pantothenic acid. No receiving-medium dose is inferred.
Stock composition (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tricine (pH 8) | 9.0 G_PER_L |
CHEBI:46760
tricine
|
Buffer adjusted to pH 8.0 |
| 2 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.67 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 3 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 4 | Vitamin B12 (Cobalamin) | 0.008 G_PER_L |
CHEBI:176843
vitamin B12
|
Source table says "add after autoclaving", but the preparation directions specify filtration without an autoclaving step. Sterilization sequence remains unresolved. |
| 5 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.02 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 6 |
4-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.04 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-03-15T00:00:00Z | mediaingredientmech-migrator-v1.0 | Created from MediaIngredientMech migration | Composition extracted from UTEX Culture Collection protocols |
| 2026-04-04T17:49:50.354211 | categorize_unknown_media | CATEGORIZED | Automated categorization (confidence: 0.11). Weak match (score: 0.13), defaulting to bacterial |
| 2026-05-16T09:02:58.417642+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | references.reference_id->reference=3 |
| 2026-05-17T07:06:06.191114+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R02_convert_exotic_units=6 |
| 2026-06-08T23:17:55.788939+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-10T07:40:20.478855+00:00 | codex | CURATED_UTEX_SEVEN_STOCK_AMOUNTS_FORMS_AND_BOUNDARIES | Source-backed amount, supplied-form and preparation-stage review. Numerical mass concentrations retained; unresolved source conflicts explicitly recorded. No taxa-media association added. |