desulfurivibrio_ames2_medium

CultureMech:015831 bacterial DEFINED LIQUID
Original name: DESULFURIVIBRIO AMeS2 MEDIUM

Overview

External ontology
jcm.grmd:1333 (source link unavailable) — DESULFURIVIBRIO AMeS2 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1333

Composition

flowchart LR
    CultureMech_015831["desulfurivibrio_ames2_medium"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_015831 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_015831 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_015831 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_015831 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Sulfur["Sulfur"]
    CultureMech_015831 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
22.0 G_PER_L CHEBI:29377
sodium carbonate
 JCM Medium 1333 lists 22.0 g Na2CO3.
2 NaHCO3
CHO3.Na 84.006 g/mol
8.0 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 JCM Medium 1333 lists 8.0 g NaHCO3.
3 NaCl
Cl.Na 58.443 g/mol
6.0 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 1333 lists 6.0 g NaCl.
4 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 1333 lists 1.0 g K2HPO4.
5 Sulfur
S 32.067 g/mol
5.0 G_PER_L CHEBI:26833
sulfur atom
 JCM Medium 1333 lists 5.0 g sulfur steamed separately before use.

Preparation

  1. Add Na2CO3, NaHCO3, NaCl, and K2HPO4 to distilled water and bring the base volume to 1.0 L.
  2. Autoclave the base in closed bottles.
  3. After cooling, aseptically add MgCl2, NH4Cl, Trace element, Se/W, and Trace vitamins stocks at the JCM Medium 1333 per-liter ratios.
  4. Steam sulfur for 3 hr on each of 3 successive days.
  5. Aseptically and anaerobically distribute the medium and sterilized sulfur into culture vessels under an N2 gas stream and seal with butyl rubber stoppers.
  6. If the inoculum does not contain sulfide or polysulfide, add 5.0 ml/L autoclaved 5% Na2S x 9H2O solution stored under N2.

Solutions

1 M MgCl2 solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgCl2 1 MOLAR Solute of the 1 M MgCl2 stock added by JCM Medium 1333.
JCM Medium 1333 adds 1.0 ml/L autoclaved 1 M MgCl2 solution.

1 M NH4Cl solution

Dose: 4.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NH4Cl 1 MOLAR Solute of the 1 M NH4Cl stock added by JCM Medium 1333.
JCM Medium 1333 adds 4.0 ml/L autoclaved 1 M NH4Cl solution.

Trace element solution (JCM Medium 1079)

Dose: 1.0 ML_PER_L

JCM Medium 1333 adds 1.0 ml/L autoclaved Trace element solution from JCM Medium 1079.

Se/W solution (JCM Medium 852)

Dose: 1.0 ML_PER_L

JCM Medium 1333 adds 1.0 ml/L filter-sterilized Se/W solution from JCM Medium 852.

Trace vitamins (JCM Medium 197)

Dose: 1.0 ML_PER_L

JCM Medium 1333 adds 1.0 ml/L filter-sterilized Trace vitamins from JCM Medium 197.

5% Na2S x 9H2O solution

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9H2O 50.0 G_PER_L Solute of the 5% Na2S x 9H2O stock added by JCM Medium 1333.
JCM Medium 1333 conditionally adds 5.0 ml/L autoclaved 5% Na2S x 9H2O solution if the inoculum lacks sulfide or polysulfide.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:08:39.276831+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1333. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.508937Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-11T00:00:00-07:00 repair_jcm_1333_desulfurivibrio_score15.py RESOLVED_JCM_1333_DESULFURIVIBRIO_SCORE15 JCM Medium 1333 lists Na2CO3, NaHCO3, NaCl, K2HPO4, and sulfur as direct components; after autoclaving the base, add MgCl2, NH4Cl, JCM 1079 Trace element, JCM 852 Se/W, and JCM 197 Trace vitamins stocks, with 5% Na2S x 9H2O solution added conditionally if the inoculum lacks sulfide or polysulfide.