Overview
- External ontology
- jcm.grmd:1334 (source link unavailable) — NATRONOARCHAEA MEDIUM II
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1334
Ingredients (0)
No ingredients recorded.
Preparation
- Mix two media autoclaved separately.
- Aseptically add the following solutions (autoclaved or *filter-sterilized): Neutral base salt medium: Add components to distilled water and bring volume to 1.0 L.
- Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.
Solutions
Neutral base salt medium
Dose: 750.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 240.0 G_PER_L | JCM Medium 1334 lists 240.0 g/L NaCl. |
| KCl | 5.0 G_PER_L | JCM Medium 1334 lists 5.0 g/L KCl. |
| K2HPO4 | 2.5 G_PER_L | JCM Medium 1334 lists 2.5 g/L K2HPO4. |
| NH4Cl | 0.4 G_PER_L | JCM Medium 1334 lists 0.4 g/L NH4Cl. |
| (NH4)2SO4 | 0.1 G_PER_L | JCM Medium 1334 lists 0.1 g/L (NH4)2SO4. |
JCM Medium 1334 adds 750.0 ml/L Neutral base salt medium prepared from the salts listed below the main recipe.
Base soda medium (JCM Medium 1207)
Dose: 250.0 ML_PER_L
JCM Medium 1334 mixes 250.0 ml/L Base soda medium.
1 M MgCl2 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 | 1.0 MOLAR | JCM Medium 1334 lists 1.0 M MgCl2. |
JCM Medium 1334 adds 1.0 ml/L 1 M MgCl2 solution.
Trace vitamins (JCM Medium 197)
Dose: 5.0 ML_PER_L
JCM Medium 1334 adds 5.0 ml/L filter-sterilized Trace vitamins.
Trace element solution (JCM Medium 1079)
Dose: 1.0 ML_PER_L
JCM Medium 1334 adds 1.0 ml/L Trace element solution.
Se/W solution (JCM Medium 852)
Dose: 0.5 ML_PER_L
JCM Medium 1334 adds 0.5 ml/L Se/W solution.
10% Yeast extract solution
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100.0 G_PER_L | JCM Medium 1334 lists 100.0 g/L Yeast extract. |
JCM Medium 1334 adds 0.2 ml/L autoclaved 10% Yeast extract solution.
10% Soluble starch solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Soluble starch | 100.0 G_PER_L | JCM Medium 1334 lists 100.0 g/L Soluble starch. |
JCM Medium 1334 adds 10.0 ml/L autoclaved 10% Soluble starch solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:08:40.327936+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1334. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.495645Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-09-13T00:00:00-07:00 | repair_jcm_score10_stock_solutions.py | RESOLVED_JCM_SCORE10_STOCK_SOLUTIONS | Resolved JCM Medium 1334's inline Neutral base salt medium and simple MgCl2, yeast extract, and soluble starch stocks; kept JCM 1207, JCM 197, JCM 1079, and JCM 852 stocks as sourced opaque cross-references. |