modified_pfennig_medium_with_acetate_and_fumarate

CultureMech:015833 bacterial DEFINED LIQUID
Original name: MODIFIED PFENNIG MEDIUM WITH ACETATE AND FUMARATE

Overview

External ontology
jcm.grmd:1341 (source link unavailable) — MODIFIED PFENNIG MEDIUM WITH ACETATE AND FUMARATE
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1341

Composition

flowchart LR
    CultureMech_015833["modified_pfennig_medium_with_acetate_and_fumarate"]
    ing0_NaCl["NaCl"]
    CultureMech_015833 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015833 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_015833 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015833 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_015833 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015833 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_Resazurin["Resazurin"]
    CultureMech_015833 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Distilled_water["Distilled water"]
    CultureMech_015833 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015833 --> ing8_8__NaHCO3_solution_
    ing9_1_0_M_Sodium_fumarate_solution["1.0 M Sodium fumarate solution"]
    CultureMech_015833 --> ing9_1_0_M_Sodium_fumarate_solution
    ing10_1_0_M_Sodium_acetate_solution["1.0 M Sodium acetate solution"]
    CultureMech_015833 --> ing10_1_0_M_Sodium_acetate_solution
    ing11_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015833 --> ing11_Trace_vitamins___see_Medium_No__197__
    ing12_Trace_element_solution___see_Medium_No__899__["Trace element solution* (see Medium No. 899 )"]
    CultureMech_015833 --> ing12_Trace_element_solution___see_Medium_No__899__
    ing13_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015833 --> ing13_5__Na2S_9H2O_solution

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.0 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
4 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 KCl
Cl.K 74.551 g/mol
0.4 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
8 Distilled water
H2O 18.015 g/mol
920.0 ML_PER_L CHEBI:15377
water
9 8% NaHCO3 solution* 50.0 ML_PER_L
10 1.0 M Sodium fumarate solution 10.0 ML_PER_L
11 1.0 M Sodium acetate solution 10.0 ML_PER_L
12 Trace vitamins* (see Medium No. 197 ) 10.0 ML_PER_L
13 Trace element solution* (see Medium No. 899 ) 1.0 ML_PER_L
14 5% Na2S·9H2O solution 6.0 ML_PER_L

Preparation

  1. Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
  2. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): Check pH to be 7.0-7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:08:41.377206+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1341. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.420439Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers