sea_water_medium_with_acetate

Overview

External ontology

jcm.grmd:1344 (source link unavailable) — SEA WATER MEDIUM WITH ACETATE

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1344

Composition

flowchart LR
    CultureMech_015834["sea_water_medium_with_acetate"]
    ing0_NaCl["NaCl"]
    CultureMech_015834 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015834 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015834 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_015834 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_015834 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_015834 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_RST_trace_elements__see_Medium_No__933__["RST trace elements (see Medium No. 933 )"]
    CultureMech_015834 --> ing6_RST_trace_elements__see_Medium_No__933__
    ing7_Resazurin["Resazurin"]
    CultureMech_015834 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Distilled_water["Distilled water"]
    CultureMech_015834 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_8_0___NaHCO3_solution_["8.0 % NaHCO3 solution*"]
    CultureMech_015834 --> ing9_8_0___NaHCO3_solution_
    ing10_RST_vitamin_solution___see_Medium_No__1097__["RST vitamin solution* (see Medium No. 1097 )"]
    CultureMech_015834 --> ing10_RST_vitamin_solution___see_Medium_No__1097__
    ing11_1_M_Sodium_acetate_solution["1 M Sodium acetate solution"]
    CultureMech_015834 --> ing11_1_M_Sodium_acetate_solution
    ing12_1__Yeast_extract_solution["1% Yeast extract solution"]
    CultureMech_015834 --> ing12_1__Yeast_extract_solution
    ing13_Reducing_agent_solution__see_Medium_No__521__["Reducing agent solution (see Medium No. 521 )"]
    CultureMech_015834 --> ing13_Reducing_agent_solution__see_Medium_No__521__

Ingredients (14)

Preparation

1. Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere.
2. After cooling, add the following solutions (autoclaved or *filter-sterilized).
3. Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance