pelosinus_bkl1_medium

Overview

External ontology

jcm.grmd:1351 (source link unavailable) — PELOSINUS BKL1 MEDIUM

Applications

• Microbial cultivation

pH

7.2

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1351

Composition

flowchart LR
    CultureMech_015835["pelosinus_bkl1_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_015835 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015835 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_NaH2PO4_2H2O["NaH2PO4·2H2O"]
    CultureMech_015835 --> ing2_NaH2PO4_2H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_2H2O -.-> CHEBI_37585
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015835 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_015835 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015835 --> ing5_Yeast_extract__BD_Difco_
    ing6_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015835 --> ing6_FeCl2_solution__see_Medium_No__187__
    ing7_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015835 --> ing7_Trace_element_solution__see_Medium_No__187__
    ing8_Distilled_water["Distilled water"]
    CultureMech_015835 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_8_0__NaHCO3_solution_["8.0% NaHCO3 solution*"]
    CultureMech_015835 --> ing9_8_0__NaHCO3_solution_
    ing10_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015835 --> ing10_Trace_vitamins___see_Medium_No__197__
    ing11_1_0_M_Sodium_lactate_solution["1.0 M Sodium lactate solution"]
    CultureMech_015835 --> ing11_1_0_M_Sodium_lactate_solution
    ing12_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015835 --> ing12_5__L_Cysteine_HCl_H2O_solution

Ingredients (13)

Preparation

1. Mix components thoroughly and adjust pH to 7.2.
2. Sparge the medium with a N2-CO2 (4:1, v/v) gas mixture and autoclave under the same gas mixture.
3. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): Readjust pH to 7.0-7.2, if necessary.

Target organisms

No target organisms are recorded.

Provenance