gbs_salts_medium_for_fervidibacter

CultureMech:015836 bacterial DEFINED LIQUID
Original name: GBS SALTS MEDIUM FOR FERVIDIBACTER

Overview

External ontology
jcm.grmd:1353 (source link unavailable) — GBS SALTS MEDIUM FOR FERVIDIBACTER
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1353

Composition

flowchart LR
    CultureMech_015836["gbs_salts_medium_for_fervidibacter"]
    ing0_NaCl["NaCl"]
    CultureMech_015836 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_015836 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Na2SO4["Na2SO4"]
    CultureMech_015836 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015836 --> ing3_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_7H2O -.-> CHEBI_31795
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015836 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_015836 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_HEPES["HEPES"]
    CultureMech_015836 --> ing6_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES -.-> CHEBI_46756
    ing7_Mineral_solution_A__see_below_["Mineral solution A (see below)"]
    CultureMech_015836 --> ing7_Mineral_solution_A__see_below_
    ing8_Distilled_water["Distilled water"]
    CultureMech_015836 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_Trace_vitamins_solution___see_below_["Trace vitamins solution* (see below)"]
    CultureMech_015836 --> ing9_Trace_vitamins_solution___see_below_
    ing10_4__Glycogen_solution["4% Glycogen solution"]
    CultureMech_015836 --> ing10_4__Glycogen_solution
    ing11_Mineral_solution_B___see_below_["Mineral solution B* (see below)"]
    CultureMech_015836 --> ing11_Mineral_solution_B___see_below_
    ing12_EDTA["EDTA"]
    CultureMech_015836 --> ing12_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing12_EDTA -.-> CHEBI_4735
    ing13_KOH["KOH"]
    CultureMech_015836 --> ing13_KOH
    CHEBI_32035["CHEBI:32035"]
    ing13_KOH -.-> CHEBI_32035
    ing14_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_015836 --> ing14_FeSO4_7H2O
    ing15_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_015836 --> ing15_MnCl2_4H2O
    ing16_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_015836 --> ing16_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_7H2O -.-> CHEBI_32312
    ing17_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_015836 --> ing17_CoCl2_6H2O
    ing18_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_015836 --> ing18_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_2H2O -.-> CHEBI_86318
    ing19_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_015836 --> ing19_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_2H2O -.-> CHEBI_75213
    ing20_H3BO3["H3BO3"]
    CultureMech_015836 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015836 --> ing21_Trace_vitamins__see_Medium_No__197__
    ing22_NaH2PO4["NaH2PO4"]
    CultureMech_015836 --> ing22_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing22_NaH2PO4 -.-> CHEBI_37585
    ing23_Na2HPO4["Na2HPO4"]
    CultureMech_015836 --> ing23_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing23_Na2HPO4 -.-> CHEBI_34683
    ing24_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_015836 --> ing24_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_2H2O -.-> CHEBI_63939
    ing25_Ni_NH4_2_SO4_2_6H2O["Ni(NH4)2(SO4)2·6H2O"]
    CultureMech_015836 --> ing25_Ni_NH4_2_SO4_2_6H2O
    ing26_VOSO4["VOSO4"]
    CultureMech_015836 --> ing26_VOSO4

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
3.0 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.15 G_PER_L CHEBI:32588
potassium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
0.3 G_PER_L CHEBI:32149
sodium sulfate
4 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.123 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
14.5 MG_PER_L CHEBI:86158
calcium chloride dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
21.4 MG_PER_L CHEBI:31206
ammonium chloride
7 HEPES 0.477 G_PER_L CHEBI:46756
HEPES
8 Mineral solution A (see below) 5.0 ML_PER_L
9 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
10 Trace vitamins solution* (see below) 10.0 ML_PER_L
11 4% Glycogen solution 10.0 ML_PER_L
12 Mineral solution B* (see below) 2.0 ML_PER_L
13 EDTA
C10H16N2O8 292.244 g/mol
2.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
14 KOH
HO.K 56.105 g/mol
2.2 G_PER_L CHEBI:32035
potassium hydroxide
15 FeSO4·7H2O 1.1 G_PER_L
16 MnCl2·4H2O 98.4 MG_PER_L
17 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
28.8 MG_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CoCl2·6H2O 23.8 MG_PER_L
19 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
17.2 MG_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
24.2 MG_PER_L CHEBI:75213
sodium molybdate dihydrate
21 H3BO3
H3BO3 61.833 g/mol
31.0 MG_PER_L CHEBI:33118
boric acid
22 Trace vitamins (see Medium No. 197 ) 1.0 ML_PER_L
23 NaH2PO4
H2O4P.Na 119.976 g/mol
0.468 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
24 Na2HPO4
HO4P.2Na 141.958 g/mol
0.866 G_PER_L CHEBI:34683
disodium hydrogenphosphate
25 Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
1.65 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 Ni(NH4)2(SO4)2·6H2O 19.8 MG_PER_L
27 VOSO4 0.82 MG_PER_L

Preparation

  1. Mix components thoroughly and adjust pH to 7.5 with NaOH.
  2. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave.
  3. After cooling, aseptically add per liter the following solutions (autoclaved or filter-sterilized): Prior to inoculation, add filter-sterilized O2 gas or air, to make a final concentration of 10% O2 in the gas phase.
  4. Mineral solution A: Adjust pH to 6.5 with HCl.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:08:44.529434+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1353. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.549100Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-15T20:07:34.008346+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 2 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Distilled water.