mineral_carbonate_medium_with_casein_peptone

CultureMech:015839 bacterial COMPLEX LIQUID
Original name: MINERAL CARBONATE MEDIUM WITH CASEIN PEPTONE

Overview

External ontology
jcm.grmd:1359 (source link unavailable) — MINERAL CARBONATE MEDIUM WITH CASEIN PEPTONE
Applications
  • Microbial cultivation
pH
9.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1359

Composition

flowchart LR
    CultureMech_015839["mineral_carbonate_medium_with_casein_peptone"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_015839 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_015839 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_015839 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_015839 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_015839 --> ing4_Yeast_extract

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
62.0 G_PER_L CHEBI:29377
sodium carbonate
 JCM Medium 1359 lists 62.0 g/L Na2CO3.
2 NaHCO3
CHO3.Na 84.006 g/mol
46.0 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 JCM Medium 1359 lists 46.0 g/L NaHCO3.
3 NaCl
Cl.Na 58.443 g/mol
18.0 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 1359 lists 18.0 g/L NaCl.
4 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 1359 lists 1.0 g/L K2HPO4.
5 Yeast extract 0.2 G_PER_L FOODON:03315426
Yeast extract
 JCM Medium 1359 lists 0.2 g/L Yeast extract.

Preparation

  1. Add components to distilled water and bring volume to 1.0 L.
  2. Adjust pH to 9.5-9.6, if necessary.
  3. Autoclave.

Solutions

1 M MgSO4 solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
MgSO4 1.0 MOLAR JCM Medium 1359 lists 1.0 M MgSO4.
JCM Medium 1359 adds 1.0 ml/L 1 M MgSO4 solution.

1 M NH4Cl solution

Dose: 4.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NH4Cl 1.0 MOLAR JCM Medium 1359 lists 1.0 M NH4Cl.
JCM Medium 1359 adds 4.0 ml/L 1 M NH4Cl solution.

Trace element solution (JCM Medium 1079)

Dose: 1.0 ML_PER_L

JCM Medium 1359 adds 1.0 ml/L Trace element solution.

Se/W solution (JCM Medium 852)

Dose: 1.0 ML_PER_L

JCM Medium 1359 adds 1.0 ml/L Se/W solution.

Trace vitamins (JCM Medium 197)

Dose: 1.0 ML_PER_L

JCM Medium 1359 adds 1.0 ml/L filter-sterilized Trace vitamins.

10% Casein peptone solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Casein peptone 100.0 G_PER_L JCM Medium 1359 lists 100.0 g/L Casein peptone.
JCM Medium 1359 adds 10.0 ml/L filter-sterilized 10% Casein peptone solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:08:47.680469+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1359. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.431534Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-13T00:00:00-07:00 repair_jcm_score10_stock_solutions.py RESOLVED_JCM_SCORE10_STOCK_SOLUTIONS Moved JCM Medium 1359 MgSO4, NH4Cl, and casein peptone stocks into solutions and grounded the direct yeast extract row.