methanobacterium_vt_medium

Overview

External ontology

jcm.grmd:1362 (source link unavailable) — METHANOBACTERIUM VT MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1362

Composition

flowchart LR
    CultureMech_015840["methanobacterium_vt_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_015840 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KCl["KCl"]
    CultureMech_015840 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015840 --> ing2_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_6H2O -.-> CHEBI_86345
    ing3_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015840 --> ing3_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_7H2O -.-> CHEBI_31795
    ing4_NH4Cl["NH4Cl"]
    CultureMech_015840 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015840 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_NaCl["NaCl"]
    CultureMech_015840 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Wolfe_s_mineral_solution__see_Medium_No__265__["Wolfe's mineral solution (see Medium No. 265 )"]
    CultureMech_015840 --> ing7_Wolfe_s_mineral_solution__see_Medium_No__265__
    ing8_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015840 --> ing8_Trace_vitamins__see_Medium_No__197__
    ing9_NaHCO3["NaHCO3"]
    CultureMech_015840 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015840 --> ing10_Yeast_extract__BD_Difco_
    ing11_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_015840 --> ing11_Trypticase_peptone__BD_BBL_
    ing12_Resazurin["Resazurin"]
    CultureMech_015840 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Distilled_water["Distilled water"]
    CultureMech_015840 --> ing13_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing13_Distilled_water -.-> CHEBI_15377
    ing14_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015840 --> ing14_5__L_Cysteine_HCl_H2O_solution
    ing15_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015840 --> ing15_5__Na2S_9H2O_solution

Ingredients (16)

Preparation

1. Mix components thoroughly, except NaHCO3, and adjust pH to 6.5.
2. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium.
3. Dispense the medium into suitable culture vessels (e.g., 5 ml medium in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers.
4. After autoclaving, stand the medium overnight.
5. Aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): Adjust pH of the medium to 6.7-7.0, if necessary.
6. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).

Target organisms

No target organisms are recorded.

Provenance