acidic_low_salt_mineral_medium

Overview

External ontology

jcm.grmd:1365 (source link unavailable) — ACIDIC LOW SALT MINERAL MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1365

Composition

flowchart LR
    CultureMech_015843["acidic_low_salt_mineral_medium"]
    ing0_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015843 --> ing0_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_7H2O -.-> CHEBI_31795
    ing1_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015843 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_K2SO4["K2SO4"]
    CultureMech_015843 --> ing2_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing2_K2SO4 -.-> CHEBI_32036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_015843 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015843 --> ing4_FeCl2_solution__see_Medium_No__187__
    ing5_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015843 --> ing5_Trace_element_solution__see_Medium_No__187__
    ing6_Selenite_tungustate_solution__see_below_["Selenite-tungustate solution (see below)"]
    CultureMech_015843 --> ing6_Selenite_tungustate_solution__see_below_
    ing7_Distilled_water["Distilled water"]
    CultureMech_015843 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_0_1_M_KH2PO4_solution["0.1 M KH2PO4 solution"]
    CultureMech_015843 --> ing8_0_1_M_KH2PO4_solution
    ing9_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015843 --> ing9_Trace_vitamins___see_Medium_No__197__
    ing10_Vitamin_B12_solution___see_Medium_No__403__["Vitamin B12 solution* (see Medium No. 403 )"]
    CultureMech_015843 --> ing10_Vitamin_B12_solution___see_Medium_No__403__
    ing11_1_0_mM_CeCl3_solution_["1.0 mM CeCl3 solution*"]
    CultureMech_015843 --> ing11_1_0_mM_CeCl3_solution_
    ing12_1_0_N_H2SO4_solution_["1.0 N H2SO4 solution*"]
    CultureMech_015843 --> ing12_1_0_N_H2SO4_solution_
    ing13_NaOH["NaOH"]
    CultureMech_015843 --> ing13_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing13_NaOH -.-> CHEBI_32145
    ing14_Na2SeO3_5H2O["Na2SeO3·5H2O"]
    CultureMech_015843 --> ing14_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing14_Na2SeO3_5H2O -.-> CHEBI_131361
    ing15_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_015843 --> ing15_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing15_Na2WO4_2H2O -.-> CHEBI_63939

Ingredients (16)

Preparation

1. Mix components thoroughly.
2. For preparation of solid medium, add 15.0 g/L agarose.
3. Autoclave and, after cooling to 60C, add the following solutions (autoclaved or *filter-sterilized): Check pH to be 2.0 to 3.0.
4. Cultivate with methane or methanol as a substrate as described below: Methane.
5. Aseptically distribute the medium into sterile serum bottles (leave ≥ 80% gas phase by volume) and seal with rubber stoppers.
6. Add sterile CO2 and methane (respectively 2-10% and 5-20% by volume) in the gas phase.
7. Cultivate with shaking.
8. Methanol: Aseptically distribute the medium into culture vessels (e.g., 10 ml in 60 ml serum bottles), supplement with 0.4-1.0 ml per liter methanol (filter-sterilized) and seal with rubber stoppers.
9. Add sterile CO2 (2-10% by volume) in the gas phase.

Target organisms

No target organisms are recorded.

Provenance