Overview
- External ontology
- jcm.grmd:1375 (source link unavailable) — MODIFIED LOW SALT MEDIUM FOR B11
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1375
Composition
flowchart LR
CultureMech_015845["modified_low_salt_medium_for_b11"]
ing0_NaCl["NaCl"]
CultureMech_015845 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_015845 --> ing1_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_6H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_015845 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NH4Cl["NH4Cl"]
CultureMech_015845 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_015845 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015845 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_Yeast_extract["Yeast extract"]
CultureMech_015845 --> ing6_Yeast_extract
ing7_Trace_element_solution__see_Medium_No__284__["Trace element solution (see Medium No. 284 )"]
CultureMech_015845 --> ing7_Trace_element_solution__see_Medium_No__284__
ing8_Resazurin["Resazurin"]
CultureMech_015845 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Distilled_water["Distilled water"]
CultureMech_015845 --> ing9_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing9_Distilled_water -.-> CHEBI_15377
ing10_8__NaHCO3_solution_["8% NaHCO3 solution*"]
CultureMech_015845 --> ing10_8__NaHCO3_solution_
ing11_10__Glucose_solution["10% Glucose solution"]
CultureMech_015845 --> ing11_10__Glucose_solution
ing12_Trace_vitamins_solution___see_Medium_No__284__["Trace vitamins solution* (see Medium No. 284 )"]
CultureMech_015845 --> ing12_Trace_vitamins_solution___see_Medium_No__284__
ing13_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
CultureMech_015845 --> ing13_5__Na2S_9H2O_solution
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
9.0 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.0 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Yeast extract | 1.0 G_PER_L | ||
| 8 | Trace element solution (see Medium No. 284 ) | 2.0 ML_PER_L | ||
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Distilled water
H2O 18.015 g/mol
|
867.0 ML_PER_L |
CHEBI:15377
water
|
|
| 11 | 8% NaHCO3 solution* | 25.0 ML_PER_L | ||
| 12 | 10% Glucose solution | 100.0 ML_PER_L | ||
| 13 | Trace vitamins solution* (see Medium No. 284 ) | 2.0 ML_PER_L | ||
| 14 | 5% Na2S·9H2O solution | 6.0 ML_PER_L |
Preparation
- Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture.
- Aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): Adjust pH to 6.5-7.0, if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:08:54.007853+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1375. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.348847Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |