eugon_agar_with_5_sheep_blood_and_artificial_seawater (CultureMech:015847)

Overview

External ontology

jcm.grmd:1380 (source link unavailable) — EUGON AGAR WITH 5% SHEEP BLOOD AND ARTIFICIAL SEAWATER

Applications

Microbial cultivation

Notes

JCM Medium 1380 combines Eugon agar, sheep blood, and artificial seawater prepared from the salts printed in JCM Medium 736.

Composition

flowchart LR
    CultureMech_015847["eugon_agar_with_5_sheep_blood_and_artificial_seawater"]
    ing0_Eugon_agar__BD_Difco_["Eugon agar (BD-Difco)"]
    CultureMech_015847 --> ing0_Eugon_agar__BD_Difco_
    ing1_Sheep_blood["Sheep blood"]
    CultureMech_015847 --> ing1_Sheep_blood

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Eugon agar (BD-Difco)	45.4 G_PER_L		JCM Medium 1380 lists 45.4 g Eugon agar (BD-Difco).
2	Sheep blood	50.0 ML_PER_L		JCM Medium 1380 lists 50.0 ml sheep blood.

Preparation

Mix Eugon agar with artificial seawater, autoclave, and cool to about 45 C.
Aseptically add 5% final sterile defibrinated sheep blood.
Mix and quickly dispense into sterile petri dishes.

Solutions

Artificial seawater

Dose: 950.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaCl	23.5 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
MgCl2 x 6 H2O	4.9 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
Na2SO4	3.9 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
CaCl2 x 2 H2O	1.1 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
KCl	0.66 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
NaHCO3	0.19 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
KBr	0.096 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
H3BO3	0.026 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
SrCl2 x 6 H2O	0.024 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
NaF	0.003 G_PER_L	JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 1380.
Distilled water	1.0 L	JCM Medium 736 lists 1.0 L distilled water for the Artificial seawater subrecipe.

JCM Medium 1380 adds 950.0 ml artificial seawater from JCM Medium 736.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-06-02T07:08:56.123574+00:00	jcm-grmd-import	Scraped from JCM GRMD database	Direct import from JCM GRMD=1380. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-09-07T00:00:00-07:00	repair_cross_reference_score25.py	RESOLVED_SCORE25_CROSS_REFERENCE	JCM Medium 1380 combines Eugon agar, sheep blood, and artificial seawater prepared from the salts printed in JCM Medium 736.