Overview
- External ontology
- jcm.grmd:1392 (source link unavailable) — ATRIBACTEROTA M15 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1392
Composition
flowchart LR
CultureMech_015850["atribacterota_m15_medium"]
ing0_NaCl["NaCl"]
CultureMech_015850 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6H2O["MgCl2 x 6H2O"]
CultureMech_015850 --> ing1_MgCl2_x_6H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6H2O -.-> CHEBI_86345
ing2_CaCl2_x_2H2O["CaCl2 x 2H2O"]
CultureMech_015850 --> ing2_CaCl2_x_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_015850 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_015850 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_015850 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Distilled_water["Distilled water"]
CultureMech_015850 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20.0 G_PER_L |
CHEBI:26710
sodium chloride
|
JCM Medium 1392 lists 20.0 g/L NaCl. |
| 2 |
MgCl2 x 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.0 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
JCM Medium 1392 lists 3.0 g/L MgCl2 x 6H2O. |
| 3 |
CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
JCM Medium 1392 lists 0.15 g/L CaCl2 x 2H2O. |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 1392 lists 0.25 g/L NH4Cl. |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 1392 lists 0.2 g/L KH2PO4. |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1.0 MG_PER_L |
CHEBI:8806
Resazurin
|
JCM Medium 1392 lists 1.0 mg/L Resazurin. |
| 7 |
Distilled water
H2O 18.015 g/mol
|
950.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1392 lists 950.0 ml/L Distilled water. |
Preparation
- Mix components thoroughly and adjust pH 7.5.
- Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream.
- Autoclave under the same gas mixture.
- After cooling, add the following solutions (filter-sterilized): Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes) under the same gas mixture and seal with butyl rubber stoppers.
- Add per liter the following solution (autoclave or *filter-sterilize and store under a N2 atmosphere).
- Readjust pH to 7.5-8.0, if necessary.
Solutions
Trace element solution (JCM Medium 439)
Dose: 1.0 ML_PER_L
JCM Medium 1392 adds 1.0 ml/L Trace element solution.
Selenite-tungstate solution (JCM Medium 431)
Dose: 1.0 ML_PER_L
JCM Medium 1392 adds 1.0 ml/L Selenite-tungstate solution.
8% NaHCO3 solution
Dose: 50.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 80.0 G_PER_L | JCM Medium 1392 lists 80.0 g/L NaHCO3. |
JCM Medium 1392 adds 50.0 ml/L 8% NaHCO3 solution.
Vitamin solution (JCM Medium 403)
Dose: 1.0 ML_PER_L
JCM Medium 1392 adds 1.0 ml/L Vitamin solution.
Thiamine solution (JCM Medium 403)
Dose: 1.0 ML_PER_L
JCM Medium 1392 adds 1.0 ml/L Thiamine solution.
Vitamin B12 solution (JCM Medium 403)
Dose: 1.0 ML_PER_L
JCM Medium 1392 adds 1.0 ml/L Vitamin B12 solution.
10% Yeast extract solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100.0 G_PER_L | JCM Medium 1392 lists 100.0 g/L Yeast extract. |
JCM Medium 1392 adds 20.0 ml/L 10% Yeast extract solution.
5% Na2S x 9H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 50.0 G_PER_L | JCM Medium 1392 lists 50.0 g/L Na2S x 9H2O. |
JCM Medium 1392 adds 10.0 ml/L 5% Na2S x 9H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:08:59.869274+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1392. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.415260Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-09-13T00:00:00-07:00 | repair_jcm_score10_stock_solutions.py | RESOLVED_JCM_SCORE10_STOCK_SOLUTIONS | Moved JCM Medium 1392 NaHCO3, yeast extract, and sulfide stocks into solutions; kept the JCM 439, JCM 431, and JCM 403 cross-referenced stocks opaque. |