Overview
- External ontology
- jcm.grmd:1395 (source link unavailable) — PFENNIG THERMODESULFOBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1395
Composition
flowchart LR
CultureMech_015852["pfennig_thermodesulfobium_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_015852 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_015852 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_KCl["KCl"]
CultureMech_015852 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015852 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_015852 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_Na2SO4["Na2SO4"]
CultureMech_015852 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_Trace_element_solution__see_Medium_No__899__["Trace element solution (see Medium No. 899 )"]
CultureMech_015852 --> ing6_Trace_element_solution__see_Medium_No__899__
ing7_Resazurin["Resazurin"]
CultureMech_015852 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Distilled_water["Distilled water"]
CultureMech_015852 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
ing9_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
CultureMech_015852 --> ing9_Trace_vitamins__see_Medium_No__197__
ing10_1_0_M_Sodium_thiosulfate_solution["1.0 M Sodium thiosulfate solution"]
CultureMech_015852 --> ing10_1_0_M_Sodium_thiosulfate_solution
ing11_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
CultureMech_015852 --> ing11_5__Na2S_9H2O_solution
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.33 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.0 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 | Trace element solution (see Medium No. 899 ) | 1.0 ML_PER_L | ||
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1.0 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Distilled water
H2O 18.015 g/mol
|
1.0 ML_PER_L |
CHEBI:15377
water
|
|
| 10 | Trace vitamins (see Medium No. 197 ) | 10.0 ML_PER_L | ||
| 11 | 1.0 M Sodium thiosulfate solution | 10.0 ML_PER_L | ||
| 12 | 5% Na2S·9H2O solution | 10.0 ML_PER_L |
Preparation
- Mix components thoroughly and adjust pH to 6.0.
- Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
- Replace the gas phase with H2- CO2 (70:30, v/v, 50 KPa overpressured).
- Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized): Readjust pH to 6.0-6.2 if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:01.987891+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1395. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.382418Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |