artificial_brackish_water_medium_2

CultureMech:015853 bacterial DEFINED LIQUID
Original name: ARTIFICIAL BRACKISH WATER MEDIUM 2

Overview

External ontology
jcm.grmd:1399 (source link unavailable) — ARTIFICIAL BRACKISH WATER MEDIUM 2
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1399

Composition

flowchart LR
    CultureMech_015853["artificial_brackish_water_medium_2"]
    ing0_NaCl["NaCl"]
    CultureMech_015853 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015853 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_015853 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KCl["KCl"]
    CultureMech_015853 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Na2SO4["Na2SO4"]
    CultureMech_015853 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_Resazurin["Resazurin"]
    CultureMech_015853 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Distilled_water["Distilled water"]
    CultureMech_015853 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
13.2 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 1399 lists 13.2 g NaCl.
2 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 1399 lists 0.2 g KH2PO4.
3 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 JCM Medium 1399 lists 0.25 g NH4Cl.
4 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
 JCM Medium 1399 lists 0.5 g KCl.
5 Na2SO4
2Na.O4S 142.043 g/mol
4.0 G_PER_L CHEBI:32149
sodium sulfate
 JCM Medium 1399 lists 4.0 g Na2SO4.
6 Resazurin
C12H7NO4 229.191 g/mol
1.0 MG_PER_L CHEBI:8806
Resazurin
 JCM Medium 1399 lists 1.0 mg resazurin.
7 Distilled water
H2O 18.015 g/mol
940.0 ML_PER_L CHEBI:15377
water
 JCM Medium 1399 lists 940.0 ml distilled water in the base.

Preparation

  1. Mix NaCl, KH2PO4, NH4Cl, KCl, Na2SO4, Trace element solution, Selenite-tungstate solution, resazurin, and distilled water.
  2. Autoclave the base under an N2-CO2 (4:1, v/v) gas atmosphere.
  3. After cooling, add bicarbonate, MgCl2 x 6H2O, CaCl2 x 2H2O, vitamin, thiamine, vitamin B12, sodium formate, and sodium acetate stocks at the JCM Medium 1399 per-liter ratios.
  4. Aseptically and anaerobically distribute 20 ml medium into 60 ml serum bottles under the same N2-CO2 gas mixture and seal with butyl rubber stoppers.
  5. Prior to use, add 6.0 ml/L autoclaved 5% Na2S x 9H2O solution stored under N2 to reduce the medium.

Solutions

Trace element solution (JCM Medium 439)

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L Trace element solution from JCM 439.

Selenite-tungstate solution (JCM Medium 431)

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L Selenite-tungstate solution from JCM 431.

8% NaHCO3 solution

Dose: 30.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaHCO3 80.0 G_PER_L Solute of the 8% NaHCO3 stock added by JCM Medium 1399.
JCM Medium 1399 adds 30.0 ml/L filter-sterilized 8% NaHCO3 solution.

1 M MgCl2 x 6H2O solution

Dose: 17.0 ML_PER_L

JCM Medium 1399 adds 17.0 ml/L anaerobic 1 M MgCl2 x 6H2O solution.

1 M CaCl2 x 2H2O solution

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L anaerobic 1 M CaCl2 x 2H2O solution.

Vitamin solution (JCM Medium 403)

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L filter-sterilized Vitamin solution.

Thiamine solution (JCM Medium 403)

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L filter-sterilized Thiamine solution.

Vitamin B12 solution (JCM Medium 403)

Dose: 1.0 ML_PER_L

JCM Medium 1399 adds 1.0 ml/L filter-sterilized Vitamin B12 solution.

1 M Sodium formate solution

Dose: 5.0 ML_PER_L

JCM Medium 1399 adds 5.0 ml/L anaerobic 1 M Sodium formate solution.

1 M Sodium acetate solution

Dose: 2.0 ML_PER_L

JCM Medium 1399 adds 2.0 ml/L anaerobic 1 M Sodium acetate solution.

5% Na2S x 9H2O solution

Dose: 6.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9H2O 50.0 G_PER_L Solute of the 5% Na2S x 9H2O stock added by JCM Medium 1399.
JCM Medium 1399 adds 6.0 ml/L autoclaved 5% Na2S x 9H2O solution before use to reduce the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:03.047606+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1399. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.556364Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-11T00:00:00-07:00 repair_jcm_1399_brackish_score15.py RESOLVED_JCM_1399_BRACKISH_SCORE15 JCM Medium 1399 base salts, resazurin, and distilled water are direct components; after autoclaving the base under N2-CO2 (4:1, v/v), add JCM 439 Trace element, JCM 431 Selenite-tungstate, JCM 403 vitamin, thiamine, and vitamin B12 stocks plus bicarbonate, magnesium chloride, calcium chloride, sodium formate, sodium acetate, and sulfide stocks.