mangrovibacterium_z1_71_medium

Overview

External ontology

jcm.grmd:1412 (source link unavailable) — MANGROVIBACTERIUM Z1-71 MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1412

Composition

flowchart LR
    CultureMech_015857["mangrovibacterium_z1_71_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015857 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015857 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015857 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_015857 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KCl["KCl"]
    CultureMech_015857 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_015857 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Wolfe_s_mineral_solution__see_Medium_No__265__["Wolfe’s mineral solution (see Medium No. 265 )"]
    CultureMech_015857 --> ing6_Wolfe_s_mineral_solution__see_Medium_No__265__
    ing7_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015857 --> ing7_Trace_vitamins__see_Medium_No__197__
    ing8_NaHCO3["NaHCO3"]
    CultureMech_015857 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015857 --> ing9_Yeast_extract__BD_Difco_
    ing10_Resazurin["Resazurin"]
    CultureMech_015857 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_015857 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377
    ing12_1_0_M_Glucose_solution["1.0 M Glucose solution"]
    CultureMech_015857 --> ing12_1_0_M_Glucose_solution
    ing13_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015857 --> ing13_5__L_Cysteine_HCl_H2O_solution
    ing14_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015857 --> ing14_5__Na2S_9H2O_solution

Ingredients (15)

Preparation

1. Mix components thoroughly, except NaHCO3, and adjust pH to 6.5.
2. Bring to a boil for 5 -10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium.
3. Dispense the medium into suitable culture vessels (e.g., 5 ml medium in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers.
4. Autoclave.
5. After autoclaving, stand the medium overnight.

Target organisms

No target organisms are recorded.

Provenance