methanosarcina_z_7115_medium (CultureMech:015858)

Overview

External ontology

jcm.grmd:1415 (source link unavailable) — METHANOSARCINA Z-7115 MEDIUM

Applications

Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1415

Composition

flowchart LR
    CultureMech_015858["methanosarcina_z_7115_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015858 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015858 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_015858 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015858 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_015858 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Trace_element_solution__see_Medium_No__899__["Trace element solution (see Medium No. 899 )"]
    CultureMech_015858 --> ing5_Trace_element_solution__see_Medium_No__899__
    ing6_Resazurin["Resazurin"]
    CultureMech_015858 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Distilled_water["Distilled water"]
    CultureMech_015858 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_8__NaHCO3_soluiton_["8% NaHCO3 soluiton*"]
    CultureMech_015858 --> ing8_8__NaHCO3_soluiton_
    ing9_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015858 --> ing9_Trace_vitamins___see_Medium_No__197__
    ing10_50___v_v__Methanol["50% (v/v) Methanol"]
    CultureMech_015858 --> ing10_50___v_v__Methanol
    ing11_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015858 --> ing11_5__L_Cysteine_HCl_H2O_solution
    ing12_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015858 --> ing12_5__Na2S_9H2O_solution

Ingredients (13)

#	Ingredient	Concentration	Ontology
1	NH4Cl Cl.H4N 53.492 g/mol	0.33 G_PER_L	CHEBI:31206 ammonium chloride
2	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	0.16 G_PER_L	CHEBI:86158 calcium chloride dihydrate
3	KH2PO4 H2O4P.K 136.084 g/mol	0.33 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
4	MgCl2·6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.33 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
5	Sodium acetate C2H3O2.Na 82.034 g/mol	0.2 G_PER_L	CHEBI:32954 sodium acetate
6	Trace element solution (see Medium No. 899 )	1.0 ML_PER_L
7	Resazurin C12H7NO4 229.191 g/mol	0.5 MG_PER_L	CHEBI:8806 Resazurin
8	Distilled water H2O 18.015 g/mol	960.0 ML_PER_L	CHEBI:15377 water
9	8% NaHCO3 soluiton*	25.0 ML_PER_L
10	*Trace vitamins (see Medium No. 197 )**	10.0 ML_PER_L
11	50% (v/v) Methanol	4.0 ML_PER_L
12	5% L-Cysteine·HCl·H2O solution	5.0 ML_PER_L
13	5% Na2S·9H2O solution	5.0 ML_PER_L

Preparation

Mix components and adjust pH to 7.0.
Autoclave the medium under a N2-CO2 (80:20 v/v) gas mixture.
After cooling, aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized): Dispense the medium into culture vessels (e.g., 20 ml of the medium in 60 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-06-02T07:09:08.381209+00:00	jcm-grmd-import	Scraped from JCM GRMD database	Direct import from JCM GRMD=1415. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.372111Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers