sporomusa_medium_2

CultureMech:015862 bacterial COMPLEX LIQUID
Original name: SPOROMUSA MEDIUM-2

Overview

External ontology
jcm.grmd:1425 (source link unavailable) — SPOROMUSA MEDIUM-2
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1425

Composition

flowchart LR
    CultureMech_015862["sporomusa_medium_2"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015862 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015862 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015862 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NaCl["NaCl"]
    CultureMech_015862 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015862 --> ing4_FeCl2_solution__see_Medium_No__187__
    ing5_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015862 --> ing5_Trace_element_solution__see_Medium_No__187__
    ing6_Se_W_solution__see_Medium_No__317__["Se/W solution (see Medium No. 317 )"]
    CultureMech_015862 --> ing6_Se_W_solution__see_Medium_No__317__
    ing7_0_1__FeSO4_7H2O_solution__in_0_1_N_H2SO4_["0.1% FeSO4·7H2O solution (in 0.1 N H2SO4)"]
    CultureMech_015862 --> ing7_0_1__FeSO4_7H2O_solution__in_0_1_N_H2SO4_
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_015862 --> ing8_Yeast_extract
    ing9_Casitone__BD_Difco_["Casitone (BD-Difco)"]
    CultureMech_015862 --> ing9_Casitone__BD_Difco_
    ing10_Resazurin["Resazurin"]
    CultureMech_015862 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_015862 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377
    ing12_K2HPO4["K2HPO4"]
    CultureMech_015862 --> ing12_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing12_K2HPO4 -.-> CHEBI_131527
    ing13_KH2PO4["KH2PO4"]
    CultureMech_015862 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_Distilled_water["Distilled water"]
    CultureMech_015862 --> ing14_Distilled_water
    ing14_Distilled_water -.-> CHEBI_15377
    ing15_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015862 --> ing15_Trace_vitamins__see_Medium_No__197__
    ing16_8__NaHCO3_solution["8% NaHCO3 solution"]
    CultureMech_015862 --> ing16_8__NaHCO3_solution
    ing17_0_1_M_Dithiothreitol_solution["0.1 M Dithiothreitol solution"]
    CultureMech_015862 --> ing17_0_1_M_Dithiothreitol_solution

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NaCl
Cl.Na 58.443 g/mol
2.25 G_PER_L CHEBI:26710
sodium chloride
5 FeCl2 solution (see Medium No. 187 ) 1.0 ML_PER_L
6 Trace element solution (see Medium No. 187 ) 1.0 ML_PER_L
7 Se/W solution (see Medium No. 317 ) 1.0 ML_PER_L
8 0.1% FeSO4·7H2O solution (in 0.1 N H2SO4) 2.0 ML_PER_L
9 Yeast extract 2.0 G_PER_L
10 Casitone (BD-Difco) 2.0 G_PER_L
11 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
12 Distilled water
H2O 18.015 g/mol
860.0 ML_PER_L CHEBI:15377
water
13 K2HPO4
HO4P.2K 174.174 g/mol
0.35 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
14 KH2PO4
H2O4P.K 136.084 g/mol
0.23 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
15 Distilled water
H2O 18.015 g/mol
100.0 ML_PER_L CHEBI:15377
water
16 Trace vitamins (see Medium No. 197 ) 10.0 ML_PER_L
17 8% NaHCO3 solution 30.0 ML_PER_L
18 0.1 M Dithiothreitol solution 10.0 ML_PER_L

Preparation

  1. Solution A: Autoclave under a N2 gas atmosphere.
  2. Solution B: Autoclave under a N2 gas atmosphere.
  3. After autoclaving, combine solutions A and B, then add the following solutions (filtersterilized): To complete the medium, aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a H2-CO2 (80:20, v/v) gas stream and seal with butyl rubber stoppers.
  4. Prior to inoculation, add per liter the following solution (freshly prepared and filter-sterilized): Adjust pH to 7.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:12.618147+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1425. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.388668Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers