modified_methanobacterium_medium (CultureMech:015863)

Overview

External ontology

jcm.grmd:1427 (source link unavailable) — MODIFIED METHANOBACTERIUM MEDIUM

Applications

Microbial cultivation

Notes

Source: JCM Medium 1427. FeCl2, trace-element, clarified-rumen-fluid, and vitamin stocks are expanded or cross-referenced from JCM Mediums 187, 266, and 898.

Composition

flowchart LR
    CultureMech_015863["modified_methanobacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_015863 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7H2O["MgSO4 x 7H2O"]
    CultureMech_015863 --> ing1_MgSO4_x_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_015863 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_015863 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2H2O["CaCl2 x 2H2O"]
    CultureMech_015863 --> ing4_CaCl2_x_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2H2O -.-> CHEBI_86158
    ing5_Brain_heart_infusion__BD_Difico_["Brain heart infusion (BD Difico)"]
    CultureMech_015863 --> ing5_Brain_heart_infusion__BD_Difico_
    ing6_Proteose_peptone__BD_Difico_["Proteose peptone (BD Difico)"]
    CultureMech_015863 --> ing6_Proteose_peptone__BD_Difico_
    ing7_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_015863 --> ing7_Yeast_extract__Oxoid_
    ing8_Sodium_acetate["Sodium acetate"]
    CultureMech_015863 --> ing8_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Sodium_acetate -.-> CHEBI_32954
    ing9_Sodium_formate["Sodium formate"]
    CultureMech_015863 --> ing9_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing9_Sodium_formate -.-> CHEBI_62965
    ing10_Resazurin["Resazurin"]
    CultureMech_015863 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_015863 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	JCM Medium 1427 lists 0.5 g/L KH2PO4.
2	MgSO4 x 7H2O 7H2O.Mg.O4S 246.473 g/mol	0.4 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	JCM Medium 1427 lists 0.4 g/L MgSO4 x 7H2O.
3	NaCl Cl.Na 58.443 g/mol	0.4 G_PER_L	CHEBI:26710 sodium chloride	JCM Medium 1427 lists 0.4 g/L NaCl.
4	NH4Cl Cl.H4N 53.492 g/mol	0.4 G_PER_L	CHEBI:31206 ammonium chloride	JCM Medium 1427 lists 0.4 g/L NH4Cl.
5	CaCl2 x 2H2O Ca.2Cl.2H2O 147.014 g/mol	0.05 G_PER_L	CHEBI:86158 calcium chloride dihydrate	JCM Medium 1427 lists 0.05 g/L CaCl2 x 2H2O.
6	Brain heart infusion (BD Difico)	6.0 G_PER_L		JCM Medium 1427 lists Brain heart infusion (BD Difico) as a commercial complex product.
7	Proteose peptone (BD Difico)	6.0 G_PER_L		JCM Medium 1427 lists Proteose peptone (BD Difico) as a complex peptone input.
8	Yeast extract (Oxoid)	2.0 G_PER_L		JCM Medium 1427 lists Yeast extract (Oxoid).
9	Sodium acetate C2H3O2.Na 82.034 g/mol	1.0 G_PER_L	CHEBI:32954 sodium acetate	JCM Medium 1427 lists 1.0 g/L Sodium acetate.
10	Sodium formate CHO2.Na 68.007 g/mol	2.0 G_PER_L	CHEBI:62965 sodium formate	JCM Medium 1427 lists 2.0 g/L Sodium formate.
11	Resazurin C12H7NO4 229.191 g/mol	0.5 MG_PER_L	CHEBI:8806 Resazurin	JCM Medium 1427 lists 0.5 mg/L Resazurin.
12	Distilled water H2O 18.015 g/mol	940.0 ML_PER_L	CHEBI:15377 water	JCM Medium 1427 lists 940.0 ml/L Distilled water.

Preparation

Mix components and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
After cooling, aseptically and anaerobically, add the following solutions from anaerobic stocks (filtersterilized): Dispense the medium into culture vessels (e.g., 5 ml medium in Hungate tubes) under a H2-CO2 (80:20, v/v) gas stream and seal with butyl stoppers.
Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): After inoculation, pressurize the inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).

Solutions

FeCl2 solution

Dose: 1.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
25% HCl (7.7 M)	10.0 ML_PER_L	JCM Medium 187 prints this component in its stock solution.
FeCl2 x 4H2O	1.5 G_PER_L	JCM Medium 187 prints this component in its stock solution.
Distilled water	990.0 ML_PER_L	JCM Medium 187 prints this component in its stock solution.

JCM Medium 1427 adds 1.0 ml/L FeCl2 solution from JCM Medium 187.

Trace element solution

Dose: 1.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
ZnCl2	70.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
MnCl2 x 4H2O	100.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
H3BO3	6.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
CoCl2 x 6H2O	190.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
CuCl2 x 2H2O	2.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
NiCl2 x 6H2O	24.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
Na2MoO4 x 2H2O	36.0 MG_PER_L	JCM Medium 187 prints this component in its stock solution.
Distilled water	1.0 L	JCM Medium 187 prints this component in its stock solution.

JCM Medium 1427 adds 1.0 ml/L Trace element solution from JCM Medium 187.

Rumen fluid, clarified

Dose: 10.0 ML_PER_L

JCM Medium 1427 adds 10.0 ml/L clarified rumen fluid prepared according to JCM Medium 266.

Vitamin solution

Dose: 1.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Vitamin B12	100.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
p-Aminobenzoic acid	80.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
Biotin	20.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
Nicotinic acid	200.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
DL-Calcium pantothenate	100.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
Pyridoxine HCl	300.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
Thiamine HCl	200.0 MG_PER_L	JCM Medium 898 prints this component in its stock solution.
Distilled water	1.0 L	JCM Medium 898 prints this component in its stock solution.

JCM Medium 1427 adds 1.0 ml/L Vitamin solution from JCM Medium 898.

8% NaHCO3 solution

Dose: 50.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaHCO3	80.0 G_PER_L	JCM Medium 1427 prints this component in its stock solution.

JCM Medium 1427 adds 50.0 ml/L filter-sterilized 8% NaHCO3 solution.

5% Na2S x 9H2O solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Na2S x 9H2O	50.0 G_PER_L	JCM Medium 1427 prints this component in its stock solution.

JCM Medium 1427 adds 10.0 ml/L 5% Na2S x 9H2O solution before inoculation.

5% L-Cysteine x HCl x H2O solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
L-Cysteine x HCl x H2O	50.0 G_PER_L	JCM Medium 1427 prints this component in its stock solution.

JCM Medium 1427 adds 10.0 ml/L 5% L-Cysteine x HCl x H2O solution before inoculation.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-06-02T07:09:13.678761+00:00	jcm-grmd-import	Scraped from JCM GRMD database	Direct import from JCM GRMD=1427. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.523456Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-09-10T00:00:00-07:00	repair_jcm_1427_methanobacterium_score15.py	RESOLVED_JCM_1427_METHANOBACTERIUM_SCORE15	Moved JCM 1427 stock-solution and cross-reference wrappers into structured solutions backed by JCM 187, 266, and 898.