m1h_nag_asw (CultureMech:015864)

Overview

External ontology

jcm.grmd:1429 (source link unavailable) — M1H NAG ASW

Applications

Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1429

Composition

flowchart LR
    CultureMech_015864["m1h_nag_asw"]
    ing0_HEPES["HEPES"]
    CultureMech_015864 --> ing0_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing0_HEPES -.-> CHEBI_46756
    ing1_Peptone["Peptone"]
    CultureMech_015864 --> ing1_Peptone
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015864 --> ing2_Yeast_extract__BD_Difco_
    ing3_Wolfe_s_mineral_solution__see_Medium_No__265__["Wolfe's mineral solution (see Medium No. 265 )"]
    CultureMech_015864 --> ing3_Wolfe_s_mineral_solution__see_Medium_No__265__
    ing4_Modified_Hutner_s_basal_salts__see_Medium_No__900__["Modified Hutner's basal salts (see Medium No. 900 )"]
    CultureMech_015864 --> ing4_Modified_Hutner_s_basal_salts__see_Medium_No__900__
    ing5_Artificial_seawater["Artificial seawater"]
    CultureMech_015864 --> ing5_Artificial_seawater
    ing6_Distilled_water["Distilled water"]
    CultureMech_015864 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Glucose["Glucose"]
    CultureMech_015864 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_N__Acetyl_D_glucosamine["N -Acetyl-D-glucosamine"]
    CultureMech_015864 --> ing8_N__Acetyl_D_glucosamine
    ing9_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
    CultureMech_015864 --> ing9_Trace_vitamins__see_Medium_No__197__
    ing10_Distilled_water["Distilled water"]
    CultureMech_015864 --> ing10_Distilled_water
    ing10_Distilled_water -.-> CHEBI_15377

Ingredients (11)

#	Ingredient	Concentration	Ontology
1	HEPES	23.8 G_PER_L	CHEBI:46756 HEPES
2	Peptone	0.25 G_PER_L	MICRO:0000178 (source link unavailable) Peptone
3	Yeast extract (BD-Difco)	0.25 G_PER_L	FOODON:03315426 Yeast extract
4	Wolfe's mineral solution (see Medium No. 265 )	1.0 ML_PER_L
5	Modified Hutner's basal salts (see Medium No. 900 )	20.0 ML_PER_L
6	Artificial seawater	250.0 ML_PER_L
7	Distilled water H2O 18.015 g/mol	729.0 ML_PER_L	CHEBI:15377 water
8	Glucose C6H12O6 180.156 g/mol	0.25 G_PER_L	CHEBI:17234 glucose
9	N -Acetyl-D-glucosamine	1.0 G_PER_L
10	Trace vitamins (see Medium No. 197 )	1.0 ML_PER_L
11	Distilled water H2O 18.015 g/mol	9.0 ML_PER_L	CHEBI:15377 water

Preparation

Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-06-02T07:09:14.733357+00:00	jcm-grmd-import	Scraped from JCM GRMD database	Direct import from JCM GRMD=1429. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.353443Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-09-13T00:00:00-07:00	repair_jcm_score10_exact_terms.py	GROUNDED_JCM_SCORE10_EXACT_TERMS	Applied local mappings for Peptone, Yeast extract (BD-Difco).